Regulator of Chromosome Condensation 1 (RCC1) (C-Term) antibody

Antigen: Regulator of Chromosome Condensation 1 (RCC1)

Synonyms: CHC1, RCC1-I, Chc1, AI326872, 4931417M11Rik, zgc:65796, zgc:77114, wu:fj77f08, SNHG3-RCC1, RCC1, MGC148464, MGC187541, rcc1, chc1-a, BJ1, CG18640 (Bj1), RanGEF, inxs, sdt, DmelCG10480, CG10480, xrcc1, chc1-b, MGC115703, MGC132085

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388624

Additional Information

Alternative name: RCC1

Gene ID: 1104

UniProt: P18754

Immunogen: This RCC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human RCC1.

Isotype: Ig

Description: Other names: Regulator of chromosome condensation, Chromosome condensation protein 1, Cell cycle regulatory protein, RCC1, CHC1,

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RCC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human RCC1.

Molecular Weight: 44969 DA

Comments:

Background: RCC1 promotes the exchange of Ran-bound GDP by GTP. It is involved in the regulation of onset of chromosome condensation in the S phase. RCC1 binds to chromatin. The RCC1/Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Patients with Raynaud disease produce antibodies that bind to RCC1.

Synonyms: CHC1, RCC1-I, Chc1, AI326872, 4931417M11Rik, zgc:65796, zgc:77114, wu:fj77f08, SNHG3-RCC1, RCC1, MGC148464, MGC187541, rcc1, chc1-a, BJ1, CG18640 (Bj1), RanGEF, inxs, sdt, DmelCG10480, CG10480, xrcc1, chc1-b, MGC115703, MGC132085

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blot~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Cycle, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of RCC1 (ABIN388624) in HL-60 cell line lysates (35ug/lane). RCC1 (arrow) was detected using the purified Pab.

Publications

Product: Nemergut, Lindsay, Brownawell et al.: "Ran-binding protein 3 links Crm1 to the Ran guanine nucleotide exchange factor." in: The Journal of biological chemistry, Vol. 277, Issue 20, pp. 17385-8, 2002 (PubMed).

Cushman, Stenoien, Moore: "The dynamic association of RCC1 with chromatin is modulated by Ran-dependent nuclear transport." in: Molecular biology of the cell, Vol. 15, Issue 1, pp. 245-55, 2003 (PubMed).

Li, Zheng: "Phosphorylation of RCC1 in mitosis is essential for producing a high RanGTP concentration on chromosomes and for spindle assembly in mammalian cells." in: Genes & development, Vol. 18, Issue 5, pp. 512-27, 2004 (PubMed).