Eukaryotic Translation Initiation Factor 2B, Subunit 3 Gamma, 58kDa (EIF2B3) (N-Term) antibody

Antigen: Eukaryotic Translation Initiation Factor 2B, Subunit 3 Gamma, 58kDa (EIF2B3)

Synonyms: MGC52809, MGC56473, zgc:56473, EIF2B3, MGC142785, NV17114

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388638

Additional Information

Alternative name: EIF2B3

Gene ID: 8891

UniProt: Q9HA31

Immunogen: This EIF2B3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human EIF2B3.

Isotype: Ig

Description: Other names: Translation initiation factor eIF-2B subunit gamma, eIF-2B GDP-GTP exchange factor subunit gamma, EIF2B3,

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This EIF2B3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human EIF2B3.

Molecular Weight: 31511 DA

Comments:

Background: EIF2B3 catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. Mutations in each of the five subunits of translation initiation factor eIF2B, including subunit 3, can cause leukoencephalopathy with vanishing white matter. EIF2B3 has also been identified as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Signaling

Restrictions: For Research Use only

Images

Western blot analysis of anti-EIF2B3 Pab (ABIN388638) in CEM (left) and Ramos (right) cell line lysates (35ug/lane). EIF2B3(arrow) was detected using the purified Pab (1:60 dilution).

Publications

Product: Gomez, Pavitt: "Identification of domains and residues within the epsilon subunit of eukaryotic translation initiation factor 2B (eIF2Bepsilon) required for guanine nucleotide exchange reveals a novel activation function promoted by eIF2B complex formation." in: Molecular and cellular biology, Vol. 20, Issue 11, pp. 3965-76, 2000 (PubMed).