Cellular Apoptosis Susceptibility (CSE1L) (N-Term) antibody

Antigen: Cellular Apoptosis Susceptibility (CSE1L)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388645

Additional Information

Gene ID: 1434

UniProt: P55060

Immunogen: This CSE1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62~92 amino acids from the N-terminal region of human CSE1L.

Isotype: Ig

Description: Other names: Exportin-2, Exp2, Importin-alpha re-exporter, Chromosome segregation 1-like protein, Cellular apoptosis susceptibility protein, CSE1L, CAS, XPO2,

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Cellular Apoptosis Susceptibility Antibody antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62~92 amino acids from the N-terminal region of human CSE1L.

Molecular Weight: 110417 DA

Comments:

Background: Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. CSE1L binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, CSE1L may play a role both in apoptosis and in cell proliferation.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-CSE1L Pab (ABIN388645) in, from left to right, A375, CEM, and mouse heart cell line lysates (35ug/lane). CSE1L(arrow) was detected using the purified Pab (1:60 dilution).

Publications

Tung, Tsai, Tung et al.: "Higher prevalence of secretory CSE1L/CAS in sera of patients with metastatic cancer." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 18, Issue 5, pp. 1570-7, 2009 (PubMed).

Stella Tsai, Chen, Tung et al.: "Serum cellular apoptosis susceptibility protein is a potential prognostic marker for metastatic colorectal cancer." in: The American journal of pathology, Vol. 176, Issue 4, pp. 1619-28, 2010 (PubMed).

Product: Wellmann, Flemming, Behrens et al.: "High expression of the proliferation and apoptosis associated CSE1L/CAS gene in hepatitis and liver neoplasms: correlation with tumor progression." in: International journal of molecular medicine, Vol. 7, Issue 5, pp. 489-94, 2001 (PubMed).

Goldberg, Alexander, Pellicena et al.: "Src phosphorylates Cas on tyrosine 253 to promote migration of transformed cells." in: The Journal of biological chemistry, Vol. 278, Issue 47, pp. 46533-40, 2003 (PubMed).