Bone Morphogenetic Protein Receptor, Type IB (BMPR1B) (C-Term) antibody

Antigen: Bone Morphogenetic Protein Receptor, Type IB (BMPR1B)

Synonyms: ALK6, ALK-6, CDw293, Alk6, Acvrlk6, BMPR-IB, CFK-43a, AI385617, AV355320, alk6, alk-6, cdw293, MGC147272, alk6b, BR1b, alk6tr, zALK-6, MGC92220, zgc:92220, BMPR1B, RPK-1, BMP15, BMPRIB, FecB

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388739

Additional Information

Alternative name: BMPR1B

Gene ID: 658

UniProt: NP_001194

Immunogen: This BMPR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 473~502 amino acids from the C-terminal region of human BMPR1B.

Isotype: Ig

Description: Other names: Serine(threonine) protein kinase, activin receptor-like kinase 6, ALK6

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BMPR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 473~502 amino acids from the C-terminal region of human BMPR1B.

Molecular Weight: 56930 DA

Comments:

Background: The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-BMPR1B Pab (ABIN388739) in NCI-H460 cell lysate. BMPR1B (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Kan, Liu, McGuire et al.: "Dysregulation of local stem/progenitor cells as a common cellular mechanism for heterotopic ossification." in: Stem cells (Dayton, Ohio), Vol. 27, Issue 1, pp. 150-6, 2009 (PubMed).

Product: ten Dijke, Yamashita, Ichijo et al.: "Characterization of type I receptors for transforming growth factor-beta and activin." in: Science (New York, N.Y.), Vol. 264, Issue 5155, pp. 101-4, 1994 (PubMed).

Lehmann, Seemann, Stricker et al.: "Mutations in bone morphogenetic protein receptor 1B cause brachydactyly type A2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 21, pp. 12277-82, 2003 (PubMed).