|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Pancreatic and Duodenal Homeobox 1 (PDX1) (N-Term) antibody
|Synonyms||GSF, IPF1, IUF1, IDX-1, MODY4, PDX-1, STF-1, PDX1, ipf1|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|3 references available|
|Quantity||400 µL (0.25 mg/ml) (Variants)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This PDX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2~32 amino acids from the N-terminal region of human PDX1.|
|Description||Other names: Pyruvate dehydrogenase protein X component, Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex, E3-binding protein|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This PDX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2~32 amino acids from the N-terminal region of human PDX1.|
|Molecular Weight||54122 DA|
Background: PDX1, located in the mitochondrial matrix, is required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. Eukaryotic pyruvate dehydrogenase complexes are organized about a core consisting of the oligomeric dihydrolipoamide acetyl-transferase, around which are arranged multiple copies of pyruvate dehydrogenase, dihydrolipoamide dehydrogenase and protein X bound by noncovalent bonds. Defects in PDHX are a cause of lacticacidemia. PDX1 belongs to the 2-oxoacid dehydrogenase family and contains 1 lipoyl-binding domain.
|Application Notes||The suggested dilution will be: ELISA~~1:1,000 Western blot~~1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Signaling, Stem Cells, Protein Modifications, Cell Structure, Metabolism|
|Restrictions||For Research Use only|
Harris, Bowker-Kinley, Wu et al.: "Dihydrolipoamide dehydrogenase-binding protein of the human pyruvate dehydrogenase complex. DNA-derived amino acid sequence, expression, and reconstitution of the pyruvate dehydrogenase complex." in: The Journal of biological chemistry, Vol. 272, Issue 32, pp. 19746-51, 1997 (PubMed).
Aral, Benelli, Ait-Ghezala et al.: "Mutations in PDX1, the human lipoyl-containing component X of the pyruvate dehydrogenase-complex gene on chromosome 11p1, in congenital lactic acidosis." in: American journal of human genetics, Vol. 61, Issue 6, pp. 1318-26, 1998 (PubMed).
Ling, McEachern, Seyda et al.: "Detection of a homozygous four base pair deletion in the protein X gene in a case of pyruvate dehydrogenase complex deficiency." in: Human molecular genetics, Vol. 7, Issue 3, pp. 501-5, 1998 (PubMed).
|Hosts||Rabbit (51), Mouse (6), Goat (4)|
|Reactivities||Human (55), Mouse (Murine) (29), Rat (Rattus) (23), Chicken (19), Pig (Porcine) (19), Chimpanzee (1), Cow (Bovine) (1), Dog (Canine) (1)|
|Applications||ELISA (34), Western Blotting (WB) (34), Immunofluorescence (IF) (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunohistochemistry (IHC) (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Flow Cytometry (FACS) (4), Dot Blot (Dot) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||N-Term (8), Internal Region (4), C-Term (2), Thr11 (2), pSer66 (2), pThr11 (2), AA 1-284 (1), AA 194-209 (1), AA 198-213 (1), AA 204-284 (1), AA 91-83 (1), Ser66 (1)|