Pancreatic and Duodenal Homeobox 1 (PDX1) (N-Term) antibody

Antigen: Pancreatic and Duodenal Homeobox 1 (PDX1)

Synonyms: GSF, IPF1, IUF1, IDX-1, MODY4, PDX-1, STF-1, PDX1, ipf1

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388772

Additional Information

Alternative name: PDX1

Gene ID: 8050

UniProt: O00330

Immunogen: This PDX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2~32 amino acids from the N-terminal region of human PDX1.

Isotype: Ig

Description: Other names: Pyruvate dehydrogenase protein X component, Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex, E3-binding protein

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This PDX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2~32 amino acids from the N-terminal region of human PDX1.

Molecular Weight: 54122 DA

Comments:

Background: PDX1, located in the mitochondrial matrix, is required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. Eukaryotic pyruvate dehydrogenase complexes are organized about a core consisting of the oligomeric dihydrolipoamide acetyl-transferase, around which are arranged multiple copies of pyruvate dehydrogenase, dihydrolipoamide dehydrogenase and protein X bound by noncovalent bonds. Defects in PDHX are a cause of lacticacidemia. PDX1 belongs to the 2-oxoacid dehydrogenase family and contains 1 lipoyl-binding domain.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blot~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Stem Cells, Protein Modifications, Cell Structure, Metabolism

Restrictions: For Research Use only

Images

Western blot analysis of hPDX1-C9 (ABIN388772) in NCI-H460 cell line lysates (35ug/lane). PDX1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Harris, Bowker-Kinley, Wu et al.: "Dihydrolipoamide dehydrogenase-binding protein of the human pyruvate dehydrogenase complex. DNA-derived amino acid sequence, expression, and reconstitution of the pyruvate dehydrogenase complex." in: The Journal of biological chemistry, Vol. 272, Issue 32, pp. 19746-51, 1997 (PubMed).

Aral, Benelli, Ait-Ghezala et al.: "Mutations in PDX1, the human lipoyl-containing component X of the pyruvate dehydrogenase-complex gene on chromosome 11p1, in congenital lactic acidosis." in: American journal of human genetics, Vol. 61, Issue 6, pp. 1318-26, 1998 (PubMed).

Ling, McEachern, Seyda et al.: "Detection of a homozygous four base pair deletion in the protein X gene in a case of pyruvate dehydrogenase complex deficiency." in: Human molecular genetics, Vol. 7, Issue 3, pp. 501-5, 1998 (PubMed).