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OCT4 antibody (POU Class 5 Homeobox 1) (AA 1-30)

Details for Product anti-POU5F1 Antibody No. ABIN388788, Supplier: Log in to see
Antigen
  • POU5F1L
  • POU5F1
  • OCT3
  • OCT4
  • OTF-3
  • OTF3
  • OTF4
  • Oct-3
  • Oct-4
  • NF-A3
  • Oct-3/4
  • Oct3
  • Oct3/4
  • Oct4
  • Otf-3
  • Otf-4
  • Otf3
  • Otf3-rs7
  • Otf3g
  • Otf4
  • gp9
  • gp-9
  • oct4
  • pou2
  • POU-2
  • cb197
  • spg/pou2
  • chunp6868
  • wu:fd18d06
  • POU class 5 homeobox 1
  • POU domain, class 5, transcription factor 1
  • POU domain, class 5, transcription factor 3
  • POU5F1
  • Pou5f1
  • pou5f3
Alternatives
anti-Human OCT4 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
AA 1-30, N-Term
44
28
25
23
21
16
15
12
9
9
6
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
372
159
72
17
16
15
14
14
9
6
5
2
2
2
1
1
1
1
1
1
Host
Rabbit
245
110
34
24
Clonality (Clone)
Polyclonal ()
Conjugate
This OCT4 antibody is un-conjugated
15
12
11
11
6
6
5
4
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
317
175
82
70
57
48
40
20
20
13
6
6
3
3
3
3
2
1
Options
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Immunogen This OCT4 (OCT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from the N-terminal region of human OCT4 (OCT3).
Clone RB3953
Isotype Ig
Specificity This OCT4 (OCT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5~34 amino acids from the N-terminal region of human 39724.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name OCT4 (OCT3) (POU5F1 Antibody Abstract)
Background Transcription factors containing the POU homeodomain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. Two forms of OCT3 mRNA are expressed in adult tissues as a result of alternative splicing--OCT3A and OCT3B. Reverse transcriptase PCR showed low level of expression in both OCT3A and OCT3B mRNA in all adult human tissues examined. Oct3 is present in mouse oocytes before and after fertilization. When fertilized oocytes were injected with antisense Oct3 oligonucleotides or double-stranded DNA containing the octamer motif, embryonic DNA synthesis was inhibited and the embryos were arrested at the one-cell stage.
Synonyms: POU domain class 5 transcription factor 1, POU5F1, Octamer-binding transcription factor 3, OCT-3, OTF3, OCT-4
Molecular Weight 38562 DA
Gene ID 5460
NCBI Accession NP_002692
Research Area Stem Cells, Transcription Factors, Signaling, Cell Structure
Pathways Stem Cell Maintenance
Application Notes WB = 1:1000, IF = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-OCT4 antibody (POU Class 5 Homeobox 1) (AA 1-30) (ABIN388788) The anti-Oct3 N-term Pab (ABIN388788) is used in Western blot to detect Oct3 in A375 ...
Immunohistochemistry (IHC) image for anti-OCT4 antibody (POU Class 5 Homeobox 1) (AA 1-30) (ABIN388788) Immunofluorescence analysis of OCT3(OCT4) antibody (N-term) (ABIN388788) in HeLa cell...
Background publications Looijenga, Stoop, de Leeuw, de Gouveia Brazao, Gillis, van Roozendaal, van Zoelen, Weber, Wolffenbuttel, van Dekken, Honecker, Bokemeyer, Perlman, Schneider, Kononen, Sauter, Oosterhuis: "POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors." in: Cancer research, Vol. 63, Issue 9, pp. 2244-50, 2003 (PubMed).

Reményi, Lins, Nissen, Reinbold, Schöler, Wilmanns: "Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers." in: Genes & development, Vol. 17, Issue 16, pp. 2048-59, 2003 (PubMed).

Crouau-Roy, Amadou, Bouissou, Clayton, Vernet, Ribouchon, Pontarotti: "Localization of the OTF3 gene within the human MHC class I region by physical and meiotic mapping." in: Genomics, Vol. 21, Issue 1, pp. 241-3, 1994 (PubMed).

Takeda, Seino, Bell: "Human Oct3 gene family: cDNA sequences, alternative splicing, gene organization, chromosomal location, and expression at low levels in adult tissues." in: Nucleic acids research, Vol. 20, Issue 17, pp. 4613-20, 1992 (PubMed).