SRY (Sex Determining Region Y)-Box 2 (SOX2) (C-Term) antibody

Antigen: SRY (Sex Determining Region Y)-Box 2 (SOX2)

Synonyms: ANOP3, MCOPS3, MGC2413, RGD1565646, SOX2, MGC148683, lcc, ysb, Sox-2

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388795

Additional Information

Alternative name: SOX2

Gene ID: 6657

UniProt: NP_003097

Immunogen: This SOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288~317 amino acids from the C-terminal region of human SOX2.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288~317 amino acids from the C-terminal region of human SOX2.

Molecular Weight: 34310 DA

Comments:

Background: This intronless gene for SOX2 encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in this gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western Blot~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of SOX2 antibody (C-term) (ABIN388795) in mouse cerebellum tissue lysates (35ug/lane). SOX2 (arrow) was detected using the purified Pab.

Publications

Product: Kamachi, Uchikawa, Kondoh: "Pairing SOX off: with partners in the regulation of embryonic development." in: Trends in genetics : TIG, Vol. 16, Issue 4, pp. 182-7, 2000 (PubMed).

Schepers, Teasdale, Koopman: "Twenty pairs of sox: extent, homology, and nomenclature of the mouse and human sox transcription factor gene families." in: Developmental cell, Vol. 3, Issue 2, pp. 167-70, 2002 (PubMed).

Fantes, Ragge, Lynch et al.: "Mutations in SOX2 cause anophthalmia." in: Nature genetics, Vol. 33, Issue 4, pp. 461-3, 2003 (PubMed).

Wiebe, Nowling, Rizzino: "Identification of novel domains within Sox-2 and Sox-11 involved in autoinhibition of DNA binding and partnership specificity." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 17901-11, 2003 (PubMed).

Reményi, Lins, Nissen et al.: "Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers." in: Genes & development, Vol. 17, Issue 16, pp. 2048-59, 2003 (PubMed).