|+1 404 474 4654|
|+1 888 205 9894 (TF)|
SRY (Sex Determining Region Y)-Box 2 (SOX2) (Cys265) antibody
|Synonyms||ANOP3, MCOPS3, MGC2413, RGD1565646, SOX2, MGC148683, lcc, ysb, Sox-2|
Alternatives Western Blotting (WB), ELISA
|5 references available|
|Quantity||400 µL (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This SOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260~289 amino acids surrounding C265 of human SOX2.|
|Description||Other names: Transcription factor SOX-2|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This SOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260~289 amino acids surrounding C265 of human SOX2.|
|Molecular Weight||34310 DA|
Background: SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation.
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Stem Cells, Transcription Factors, Signaling|
|Restrictions||For Research Use only|
|Western blot analysis of SOX2 (arrow) using rabbit polyclonal SOX2 Antibody (C265) (ABIN388798). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies).|
Kamachi, Uchikawa, Kondoh: "Pairing SOX off: with partners in the regulation of embryonic development." in: Trends in genetics : TIG, Vol. 16, Issue 4, pp. 182-7, 2000 (PubMed).
Schepers, Teasdale, Koopman: "Twenty pairs of sox: extent, homology, and nomenclature of the mouse and human sox transcription factor gene families." in: Developmental cell, Vol. 3, Issue 2, pp. 167-70, 2002 (PubMed).
Fantes, Ragge, Lynch et al.: "Mutations in SOX2 cause anophthalmia." in: Nature genetics, Vol. 33, Issue 4, pp. 461-3, 2003 (PubMed).
Wiebe, Nowling, Rizzino: "Identification of novel domains within Sox-2 and Sox-11 involved in autoinhibition of DNA binding and partnership specificity." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 17901-11, 2003 (PubMed).
Reményi, Lins, Nissen et al.: "Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers." in: Genes & development, Vol. 17, Issue 16, pp. 2048-59, 2003 (PubMed).