SLUG (Center) antibody

Antigen: SLUG

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388807

Additional Information

Gene ID: 6591

UniProt: O43623

Immunogen: This SLUG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107~136 amino acids from the central region of human SLUG.

Isotype: Ig

Description: Other names: Zinc finger protein SLUG, Neural crest transcription factor Slug, Snail homolog 2,SNAI2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SLUG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107~136 amino acids from the central region of human SLUG.

Molecular Weight: 29986 DA

Comments:

Background: SLUG is a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporadic cases of neural tube defects.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Cancer, Transcription Factors, Signaling

Restrictions: For Research Use only

Images

SLUG Antibody (Center) (ABIN388807) is used in Western blot to detect SLUG in mouse heart tissue lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human Breast tissue reacted with SLUG Antibody (Center)(ABIN388807), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Hemavathy, Guru, Harris et al.: "Human Slug is a repressor that localizes to sites of active transcription." in: Molecular and cellular biology, Vol. 20, Issue 14, pp. 5087-95, 2000 (PubMed).

Hajra, Chen, Fearon: "The SLUG zinc-finger protein represses E-cadherin in breast cancer." in: Cancer research, Vol. 62, Issue 6, pp. 1613-8, 2002 (PubMed).

Sánchez-Martín, Rodríguez-García, Pérez-Losada et al.: "SLUG (SNAI2) deletions in patients with Waardenburg disease." in: Human molecular genetics, Vol. 11, Issue 25, pp. 3231-6, 2002 (PubMed).