Growth Differentiation Factor 15 (GDF15) (N-Term) antibody

Antigen: Growth Differentiation Factor 15 (GDF15)

Synonyms: GDF15, PDF, MIC1, PLAB, MIC-1, NAG-1, PTGFB, GDF-15, SBF

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388815

Additional Information

Alternative name: GDF15

Gene ID: 9518

UniProt: GDF15_HUMAN

Immunogen: This GDF15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23~52 amino acids from the N-terminal region of human GDF15.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GDF15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23~52 amino acids from the N-terminal region of human GDF15.

Molecular Weight: 34168 DA

Comments:

Background: GDF15 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. GDF15 is highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Stem Cells, Cell Signaling, Cell Structure

Restrictions: For Research Use only

Images

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with GDF15 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Western blot analysis of GDF15 (arrow) using rabbit polyclonal GDF15 Antibody (L30) (ABIN388815). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GDF15 gene.

Publications

Product: Bootcov, Bauskin, Valenzuela et al.: "MIC-1, a novel macrophage inhibitory cytokine, is a divergent member of the TGF-beta superfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 21, pp. 11514-9, 1997 (PubMed).

Hromas, Hufford, Sutton et al.: "PLAB, a novel placental bone morphogenetic protein." in: Biochimica et biophysica acta, Vol. 1354, Issue 1, pp. 40-4, 1997 (PubMed).

Paralkar, Vail, Grasser et al.: "Cloning and characterization of a novel member of the transforming growth factor-beta/bone morphogenetic protein family." in: The Journal of biological chemistry, Vol. 273, Issue 22, pp. 13760-7, 1998 (PubMed).