Ariadne Homolog, Ubiquitin-Conjugating Enzyme E2 Binding Protein, 1 (Drosophila) (ARIH1) (C-Term) antibody

Antigen: Ariadne Homolog, Ubiquitin-Conjugating Enzyme E2 Binding Protein, 1 (Drosophila) (ARIH1)

Synonyms: Ari, Ari1, Hari, Hhari, Uip77, Ubch7bp, AU021774, ARI, HARI, HHARI, UBCH7BP, FLJ20329, FLJ93118, DKFZp686O13120, zgc:66364, wu:fa18d01, ARIH1, MGC148912, ariadne, MGC130861, ari, hari, hhari, ubch7bp

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388823

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: ARIH1 (UbcH7-BP) (C-term)

Gene ID: 25820

Swiss-Prot: Q9Y4X5

Immunogen: This ARIH1 (UbcH7-BP) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 525~555 amino acids from the C-terminal region of human UbcH7-BP.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Protein ariadne-1 homolog, ARI-1, Ubiquitin-conjugating enzyme E2-binding protein 1, UbcH7-binding protein, UbcM4-interacting protein, HHARI, H7-AP2, Monocyte protein 6, MOP-6, ARIH1, ARI, MOP6, UBCH7BP

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UbcH7-BP.

Molecular Weight: 64118 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blot~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Translation Factors, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle, Transcription Factors, Signaling, Neurology

Restrictions: For Research Use only

Images

Western blot analysis of UbcH7(arrow) using rabbit polyclonal UbcH7-BP Antibody (C-term) (ABIN388823). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ARIH1 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.