|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5) (C-Term) antibody
|Synonyms||DD5, EDD, HYD, EDD1, FLJ11310, KIAA0896, MGC57263|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|4 references available|
|Quantity||400 µL (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This EDD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2745~2775 amino acids from the C-terminal region of human EDD.|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This EDD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2745~2775 amino acids from the C-terminal region of human EDD.|
|Molecular Weight||309348 DA|
Background: EDD is a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22, a locus disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Ubiquitin-related antibodies, Protein Modifications, Cancer, Cell Cycle, Signaling, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Callaghan, Russell, Woollatt et al.: "Identification of a human HECT family protein with homology to the Drosophila tumor suppressor gene hyperplastic discs." in: Oncogene, Vol. 17, Issue 26, pp. 3479-91, 1999 (PubMed).
Honda, Tojo, Matsuzaki et al.: "Cooperation of HECT-domain ubiquitin ligase hHYD and DNA topoisomerase II-binding protein for DNA damage response." in: The Journal of biological chemistry, Vol. 277, Issue 5, pp. 3599-605, 2002 (PubMed).
Henderson, Russell, Hird et al.: "EDD, the human hyperplastic discs protein, has a role in progesterone receptor coactivation and potential involvement in DNA damage response." in: The Journal of biological chemistry, Vol. 277, Issue 29, pp. 26468-78, 2002 (PubMed).
Clancy, Henderson, Russell et al.: "EDD, the human orthologue of the hyperplastic discs tumour suppressor gene, is amplified and overexpressed in cancer." in: Oncogene, Vol. 22, Issue 32, pp. 5070-81, 2003 (PubMed).
|Hosts||Rabbit (10), Goat (5), Sheep (1)|
|Reactivities||Human (13), Mouse (Murine) (3), Cow (Bovine) (2), Pig (Porcine) (1), Rabbit (1), Rat (Rattus) (1)|
|Applications||ELISA (12), Western Blotting (WB) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunohistochemistry (IHC) (3), Immunoprecipitation (IP) (2)|
|Epitopes||C-Term (4), Center (2), AA 1-227 (1), AA 600-650 (1), Internal Region (1), N-Term (1)|