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Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5) (C-Term) antibody

Details for Product No. ABIN388824
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Antigen
Epitope
C-Term
(13), (6), (4), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(55), (32), (17), (15), (12), (12), (3), (3)
Host
Rabbit
(44), (8), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(35), (33), (21), (17), (10), (5), (2), (1)
Pubmed 4 references available
Quantity 400 µL
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Catalog No. ABIN388824
291.50 $
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  • +1 404 474 4654
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Immunogen This EDD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2745~2775 amino acids from the C-terminal region of human EDD.
Specificity This EDD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2745~2775 amino acids from the C-terminal region of human EDD.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name EDD
Background EDD is a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22, a locus disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation.
Molecular Weight 309348 DA
Gene ID 51366
UniProt O95071
Research Area Ubiquitin-related antibodies, Protein Modifications, Cancer, Cell Cycle, Signaling, Chromatin, Cell Structure
Application Notes Western blotting=1:100-500, Immunohistochemistry=1:50-100
Comment

Background: EDD is a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22, a locus disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation.

Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5) (C-Term) antibody Western blot analysis of anti-EDD Pab (ABIN388824) in, from left to right, mouse testis, mouse brain, and Jurkat cell line lysate (35 µg/lane). EDD(arrow) was detected using the purified polyclonal antibody (1:60 dilution)
anti-Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5) (C-Term) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Callaghan, Russell, Woollatt et al.: "Identification of a human HECT family protein with homology to the Drosophila tumor suppressor gene hyperplastic discs." in: Oncogene, Vol. 17, Issue 26, pp. 3479-91, 1999 (PubMed).

Honda, Tojo, Matsuzaki et al.: "Cooperation of HECT-domain ubiquitin ligase hHYD and DNA topoisomerase II-binding protein for DNA damage response." in: The Journal of biological chemistry, Vol. 277, Issue 5, pp. 3599-605, 2002 (PubMed).

Henderson, Russell, Hird et al.: "EDD, the human hyperplastic discs protein, has a role in progesterone receptor coactivation and potential involvement in DNA damage response." in: The Journal of biological chemistry, Vol. 277, Issue 29, pp. 26468-78, 2002 (PubMed).

Clancy, Henderson, Russell et al.: "EDD, the human orthologue of the hyperplastic discs tumour suppressor gene, is amplified and overexpressed in cancer." in: Oncogene, Vol. 22, Issue 32, pp. 5070-81, 2003 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN388824
291.50 $
Plus shipping costs $45.00
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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