Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5) (C-Term) antibody

Antigen: Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5)

Synonyms: DD5, EDD, HYD, EDD1, FLJ11310, KIAA0896, MGC57263

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388824

Additional Information

Alternative name: EDD

Gene ID: 51366

UniProt: O95071

Immunogen: This EDD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2745~2775 amino acids from the C-terminal region of human EDD.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This EDD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2745~2775 amino acids from the C-terminal region of human EDD.

Molecular Weight: 309348 DA

Comments:

Background: EDD is a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22, a locus disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Cancer, Cell Cycle, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-EDD Pab (ABIN388824) in, from left to right, mouse testis, mouse brain, and Jurkat cell line lysate (35ug/lane). EDD(arrow) was detected using the purified Pab (1:60 dilution)

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Callaghan, Russell, Woollatt et al.: "Identification of a human HECT family protein with homology to the Drosophila tumor suppressor gene hyperplastic discs." in: Oncogene, Vol. 17, Issue 26, pp. 3479-91, 1999 (PubMed).

Honda, Tojo, Matsuzaki et al.: "Cooperation of HECT-domain ubiquitin ligase hHYD and DNA topoisomerase II-binding protein for DNA damage response." in: The Journal of biological chemistry, Vol. 277, Issue 5, pp. 3599-605, 2002 (PubMed).

Henderson, Russell, Hird et al.: "EDD, the human hyperplastic discs protein, has a role in progesterone receptor coactivation and potential involvement in DNA damage response." in: The Journal of biological chemistry, Vol. 277, Issue 29, pp. 26468-78, 2002 (PubMed).

Clancy, Henderson, Russell et al.: "EDD, the human orthologue of the hyperplastic discs tumour suppressor gene, is amplified and overexpressed in cancer." in: Oncogene, Vol. 22, Issue 32, pp. 5070-81, 2003 (PubMed).