Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5) (C-Term) antibody

Details for Product No. ABIN388824
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Antigen
Synonyms DD5, EDD, EDD1, HYD, 4432411E13Rik, AW549941, C77315, D030042K14, Edd, Edd1, mKIAA0896, Dd5, Rat100
Epitope
C-Term
(13), (6), (4), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(55), (32), (17), (15), (12), (12), (3), (3)
Host
Rabbit
(44), (8), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(35), (33), (21), (17), (10), (5), (2), (1)
Pubmed 4 references available
Catalog no. ABIN388824
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Immunogen This EDD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2745~2775 amino acids from the C-terminal region of human EDD.
Specificity This EDD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2745~2775 amino acids from the C-terminal region of human EDD.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name EDD
Background EDD is a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22, a locus disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation.
Molecular Weight 309348 DA
Gene ID 51366
UniProt O95071
Research Area Ubiquitin-related antibodies, Protein Modifications, Cancer, Cell Cycle, Signaling, Chromatin, Cell Structure
Application Notes Western blotting=1:100-500, Immunohistochemistry=1:50-100
Comment

Background: EDD is a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22, a locus disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation.

Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5) (C-Term) antibody Western blot analysis of anti-EDD Pab (ABIN388824) in, from left to right, mouse testis, mouse brain, and Jurkat cell line lysate (35 µg/lane). EDD(arrow) was detected using the purified polyclonal antibody (1:60 dilution)
anti-Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5) (C-Term) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Callaghan, Russell, Woollatt et al.: "Identification of a human HECT family protein with homology to the Drosophila tumor suppressor gene hyperplastic discs." in: Oncogene, Vol. 17, Issue 26, pp. 3479-91, 1999 (PubMed).

Honda, Tojo, Matsuzaki et al.: "Cooperation of HECT-domain ubiquitin ligase hHYD and DNA topoisomerase II-binding protein for DNA damage response." in: The Journal of biological chemistry, Vol. 277, Issue 5, pp. 3599-605, 2002 (PubMed).

Henderson, Russell, Hird et al.: "EDD, the human hyperplastic discs protein, has a role in progesterone receptor coactivation and potential involvement in DNA damage response." in: The Journal of biological chemistry, Vol. 277, Issue 29, pp. 26468-78, 2002 (PubMed).

Clancy, Henderson, Russell et al.: "EDD, the human orthologue of the hyperplastic discs tumour suppressor gene, is amplified and overexpressed in cancer." in: Oncogene, Vol. 22, Issue 32, pp. 5070-81, 2003 (PubMed).

Hosts (44), (8), (1), (1)
Reactivities (55), (32), (17), (15), (12), (12), (3), (3)
Applications (35), (33), (21), (17), (10), (5), (2), (1)
Conjugates (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (13), (6), (4), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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