SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (N-Term), (AA 6-36) antibody

Details for Product No. ABIN388830
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Antigen
Synonyms SMURF2, 2810411E22Rik, AI558114, AI649275, RGD1310067, wu:fb82a11, wu:fc41c02
Epitope
N-Term, AA 6-36
(13), (8), (6), (2), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(41), (17), (13), (12), (12)
Host
Rabbit
(40), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(32), (14), (13), (10), (10), (8), (2), (2), (2)
Pubmed 3 references available
Quantity 400 μL
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Catalog No. ABIN388830
291.50 $
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Immunogen This SMURF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-36 AA from the N-terminal region of human SMURF2.
Clone RB04106
Isotype Ig
Specificity This SMURF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13~43 amino acids from the N-terminal region of human SMURF2.
Predicted Reactivity Zebrafish
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name SMURF2
Background SMURF2, a 748-amino acid ubiquitin E3 ligase that is 83% identical to SMURF1, codes for a C2-WW-HECT domain ubiquitin ligase that associates constitutively with SMAD7. Binding to SMAD7 induces export of SMURF2 and recruitment to the activated transforming growth factor-beta receptor (TGFBR), where it causes receptor and SMAD7 degradation. A strong interaction of second and third SMURF2 WW domains has been identified with SMAD1, SMAD2, and SMAD3, but not SMAD4. Western blot analysis showed that SMURF2 selectively downregulates the transcription of SMAD2 and SMAD1, but not SMAD3. The nuclear SMURF2/phosphorylated SMAD2 interaction is requires TGFB1.
Synonyms: Smad ubiquitination regulatory factor 2, Ubiquitin--protein ligase SMURF2, Smad-specific E3 ubiquitin ligase 2, hSMURF2
Molecular Weight 86195 DA
Gene ID 64750
NCBI Accession NP_073576
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (N-Term), (AA 6-36) antibody The anti-SMURF2 Pab (ABIN388830) is used in Western blot to detect SMURF2 in mouse brain tissue lysate.
anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (N-Term), (AA 6-36) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Lin, Liang, Feng: "Smurf2 is a ubiquitin E3 ligase mediating proteasome-dependent degradation of Smad2 in transforming growth factor-beta signaling." in: The Journal of biological chemistry, Vol. 275, Issue 47, pp. 36818-22, 2001 (PubMed).

Zhang, Chang, Gehling et al.: "Regulation of Smad degradation and activity by Smurf2, an E3 ubiquitin ligase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 3, pp. 974-9, 2001 (PubMed).

Kavsak, Rasmussen, Causing et al.: "Smad7 binds to Smurf2 to form an E3 ubiquitin ligase that targets the TGF beta receptor for degradation." in: Molecular cell, Vol. 6, Issue 6, pp. 1365-75, 2001 (PubMed).

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