SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (N-Term) antibody

Antigen: SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2)

Synonyms: MGC138150, DKFZp686F0270, AI558114, AI649275, 2810411E22Rik, RGD1310067, wu:fb82a11, wu:fc41c02, LOC398372, smurf2, MGC130978, SMURF2

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388830

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: SMURF2 (N-term)

Gene ID: 64750

Swiss-Prot: NP_073576

Immunogen: This SMURF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13~43 amino acids from the N-terminal region of human SMURF2.

Isotype: Ig

Description: Other names: Smad ubiquitination regulatory factor 2, Ubiquitin--protein ligase SMURF2, Smad-specific E3 ubiquitin ligase 2, hSMURF2

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SMURF2.

Molecular Weight: 86195 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

The anti-SMURF2 Pab (ABIN388830) is used in Western blot to detect SMURF2 in mouse brain tissue lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.