You are viewing an incomplete version of our website. Please click to reload the website as full version.

Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1) (AA 111-140), (C-Term) antibody

Details for Product No. ABIN388843, Supplier: Login to see
Antigen
  • e2(17)kb1
  • sft
  • ubc4/5
  • ubch5
  • ubch5a
  • E2(17)KB1
  • SFT
  • UBC4/5
  • UBCH5
  • UBCH5A
  • ube2d1
  • wu:fc16h06
  • zgc:73096
Epitope
AA 111-140, C-Term
20
8
3
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
61
26
22
3
1
1
1
1
1
Host
Rabbit
52
9
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
44
26
24
11
11
1
1
1
Supplier
Login to see
Supplier Product No.
Login to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Available images

Immunogen This UBE2D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 AA from the C-terminal region of human UBE2D1.
Clone RB04139
Isotype Ig
Specificity This UBE2D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118~147 amino acids from the C-terminal region of human UBE2D1.
Predicted Reactivity Cow (Bovine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name UBE2D1 (UBE2D1 Antibody Abstract)
Background The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D1 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.
Synonyms: Ubiquitin-conjugating enzyme E2-17 kDa 1, Ubiquitin-protein ligase, Ubiquitin carrier protein, E2(17)KB 1
Molecular Weight 16602 DA
Gene ID 7321
NCBI Accession NP_003329
Research Area Ubiquitin-related antibodies, Protein Modifications, Cancer, Transcription Factors, Signaling, Cell Structure
Pathways
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1) (AA 111-140), (C-Term) antibody (ABIN388843) Western blot analysis of anti-UBE2D1 Pab (ABIN388843) in mouse cerebellum tissue lysa...
Western Blotting (WB) image for anti-Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1) (AA 111-140), (C-Term) antibody (ABIN388843) Western blot analysis of anti-UBE2D1 Pab (ABIN388843) in, left-to-right, wild-type T3...
Immunohistochemistry (IHC) image for anti-Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1) (AA 111-140), (C-Term) antibody (ABIN388843) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Background publications Brès, Kiernan, Linares et al.: "A non-proteolytic role for ubiquitin in Tat-mediated transactivation of the HIV-1 promoter." in: Nature cell biology, Vol. 5, Issue 8, pp. 754-61, 2003 (PubMed).

Gehrke, Riedel, Herrmann et al.: "UbcH5A, a member of human E2 ubiquitin-conjugating enzymes, is closely related to SFT, a stimulator of iron transport, and is up-regulated in hereditary hemochromatosis." in: Blood, Vol. 101, Issue 8, pp. 3288-93, 2003 (PubMed).

Kamura, Sato, Iwai et al.: "Activation of HIF1alpha ubiquitination by a reconstituted von Hippel-Lindau (VHL) tumor suppressor complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 19, pp. 10430-5, 2000 (PubMed).

Jensen, Bates, Yang et al.: "Identification of a family of closely related human ubiquitin conjugating enzymes." in: The Journal of biological chemistry, Vol. 270, Issue 51, pp. 30408-14, 1996 (PubMed).