Ubiquitin-Conjugating Enzyme E2N (UBE2N) (N-Term) antibody

Antigen: Ubiquitin-Conjugating Enzyme E2N (UBE2N)

Synonyms: UBC13, AL022654, BB101821, 1500026J17Rik, MGC93937, ube2n, MGC64428, zgc:55726, wu:fb11b03, wu:fc08b06, UBE2N, MGC139855, ubc13, MGC75672, ubch-ben

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388867

Additional Information

Alternative name: UBE2N

Gene ID: 7334

UniProt: NP_003339

Immunogen: This UBE2N antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3~32 amino acids from the N-terminal region of human UBE2N.

Isotype: Ig

Description: Other names: Ubiquitin-conjugating enzyme E2 N, Ubiquitin-protein ligase N, Ubiquitin carrier protein N, Ubc13, Bendless-like ubiquitin conjugating enzyme

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This UBE2N antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3~32 amino acids from the N-terminal region of human UBE2N.

Molecular Weight: 17138 DA

Comments:

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2N is a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-UBE2N Antibody (N-term) (ABIN388867) in CEM cell line lysates (35ug/lane). UBE2N(arrow) was detected using the purified Pab.

Publications

Product: Yamaguchi, Kim, Sekine et al.: "Cloning and expression of cDNA encoding a human ubiquitin-conjugating enzyme similar to the Drosophila bendless gene product." in: Journal of biochemistry, Vol. 120, Issue 3, pp. 494-97, 1997 (PubMed).

McKenna, Moraes, Pastushok et al.: "An NMR-based model of the ubiquitin-bound human ubiquitin conjugation complex Mms2.Ubc13. The structural basis for lysine 63 chain catalysis." in: The Journal of biological chemistry, Vol. 278, Issue 15, pp. 13151-8, 2003 (PubMed).

Bothos, Summers, Venere et al.: "The Chfr mitotic checkpoint protein functions with Ubc13-Mms2 to form Lys63-linked polyubiquitin chains." in: Oncogene, Vol. 22, Issue 46, pp. 7101-7, 2003 (PubMed).