Toll Interacting Protein (TOLLIP) (C-Term) antibody

Antigen: Toll Interacting Protein (TOLLIP)

Synonyms: FLJ33531, IL-1RAcPIP, 4930403G24Rik, 4931428G15Rik, fk22a02, zgc:76985, wu:fk22a02, tollip, MGC79626, TOLLIP, MGC139368, GB17961

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388940

Additional Information

Alternative name: TOLLIP

Gene ID: 54472

UniProt: Q9H0E2

Immunogen: This TOLLIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228~258 amino acids from the C-terminal region of human TOLLIP.

Isotype: Ig

Description: Other names: Toll-interacting protein

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This TOLLIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228~258 amino acids from the C-terminal region of human TOLLIP.

Molecular Weight: 30282 DA

Comments:

Background: Toll like protein is a component of the signaling pathway of IL1 and Toll like receptors. It inhibits cell activation by microbial products. Tollip recruits IRAK1 to the IL1 receptor complex and inhibits IRAK1 phosphorylation and kinase activity. It oligomerizes and binds to TLR2 and the TLR4-MD2 complex via its C terminus. It exists as a complex with IRAK1 in unstimulated cells. Upon IL1 signaling, Tollip binds to the activated IL1 receptor complex containing IL-1RI, IL-1RacP and the adapter protein MyD88, where it interacts with the TIR domain of IL-1RacP. MyD88 then triggers IRAK1 autophosphorylation, which in turn leads to the dissociation of IRAK1 from Tollip and IL-1RAcP. TOLLIP also interacts with TLR2 and TLR4, TOLLIP overexpression inhibits nuclear factor kappa-B (NFKB) activation in response to lipopolysaccharide and IL1B.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Middle panels, TOLLIP expression in matched normal and tumor breast samples. Here the TOLLIP stain in the invasive ductal carcinoma cells shows a much stronger signal compared with the normal ductal cells. Bottom panels, TOLLIP expression in matched normal and tumor breast samples. In this case, the signal for TOLLIP in the normal ductal cells is stronger than that in the invasive ductal carcinoma cells.

Western blot analysis of TOLLIP (arrow) using rabbit polyclonal TOLLIP Antibody (A236) (ABIN388940). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TOLLIP gene.

Publications

Product: Volpe, Clatworthy, Kaptein et al.: "The IL1 receptor accessory protein is responsible for the recruitment of the interleukin-1 receptor associated kinase to the IL1/IL1 receptor I complex." in: FEBS letters, Vol. 419, Issue 1, pp. 41-4, 1998 (PubMed).

Burns, Clatworthy, Martin et al.: "Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor." in: Nature cell biology, Vol. 2, Issue 6, pp. 346-51, 2000 (PubMed).

Bulut, Faure, Thomas et al.: "Cooperation of Toll-like receptor 2 and 6 for cellular activation by soluble tuberculosis factor and Borrelia burgdorferi outer surface protein A lipoprotein: role of Toll-interacting protein and IL-1 receptor signaling molecules in Toll-like receptor 2 s" in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 2, pp. 987-94, 2001 (PubMed).

Zhang, Ghosh: "Negative regulation of toll-like receptor-mediated signaling by Tollip." in: The Journal of biological chemistry, Vol. 277, Issue 9, pp. 7059-65, 2002 (PubMed).