|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Toll Interacting Protein (TOLLIP) (C-Term) antibody
|Synonyms||FLJ33531, IL-1RAcPIP, 4930403G24Rik, 4931428G15Rik, fk22a02, zgc:76985, wu:fk22a02, tollip, MGC79626, TOLLIP, MGC139368, GB17961|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|4 references available|
|Quantity||400 µL (0.25 mg/ml) (Variants)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This TOLLIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228~258 amino acids from the C-terminal region of human TOLLIP.|
|Description||Other names: Toll-interacting protein|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This TOLLIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228~258 amino acids from the C-terminal region of human TOLLIP.|
|Molecular Weight||30282 DA|
Background: Toll like protein is a component of the signaling pathway of IL1 and Toll like receptors. It inhibits cell activation by microbial products. Tollip recruits IRAK1 to the IL1 receptor complex and inhibits IRAK1 phosphorylation and kinase activity. It oligomerizes and binds to TLR2 and the TLR4-MD2 complex via its C terminus. It exists as a complex with IRAK1 in unstimulated cells. Upon IL1 signaling, Tollip binds to the activated IL1 receptor complex containing IL-1RI, IL-1RacP and the adapter protein MyD88, where it interacts with the TIR domain of IL-1RacP. MyD88 then triggers IRAK1 autophosphorylation, which in turn leads to the dissociation of IRAK1 from Tollip and IL-1RAcP. TOLLIP also interacts with TLR2 and TLR4, TOLLIP overexpression inhibits nuclear factor kappa-B (NFKB) activation in response to lipopolysaccharide and IL1B.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca|
|Research Area||Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cell Structure|
|Restrictions||For Research Use only|
Volpe, Clatworthy, Kaptein et al.: "The IL1 receptor accessory protein is responsible for the recruitment of the interleukin-1 receptor associated kinase to the IL1/IL1 receptor I complex." in: FEBS letters, Vol. 419, Issue 1, pp. 41-4, 1998 (PubMed).
Burns, Clatworthy, Martin et al.: "Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor." in: Nature cell biology, Vol. 2, Issue 6, pp. 346-51, 2000 (PubMed).
Bulut, Faure, Thomas et al.: "Cooperation of Toll-like receptor 2 and 6 for cellular activation by soluble tuberculosis factor and Borrelia burgdorferi outer surface protein A lipoprotein: role of Toll-interacting protein and IL-1 receptor signaling molecules in Toll-like receptor 2 s" in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 2, pp. 987-94, 2001 (PubMed).
Zhang, Ghosh: "Negative regulation of toll-like receptor-mediated signaling by Tollip." in: The Journal of biological chemistry, Vol. 277, Issue 9, pp. 7059-65, 2002 (PubMed).
|Hosts||Rabbit (44), Mouse (12), Rat (4)|
|Reactivities||Human (53), Mouse (Murine) (26), Rat (Rattus) (19), Cow (Bovine) (2), Xenopus laevis (2), Zebrafish (2), Chicken (1), Fish (1)|
|Applications||Western Blotting (WB) (57), ELISA (29), Immunohistochemistry (IHC) (15), Immunocytochemistry (ICC) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Immunoprecipitation (IP) (6), Immunofluorescence (IF) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Flow Cytometry (FACS) (2)|
|Epitopes||C-Term (11), N-Term (5), Internal Region (4), AA 61-274 (2), AA 1-274 (1), Center (1), Middle Region (1), internal (1)|