|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Toll Interacting Protein (TOLLIP) (Center) antibody
|Synonyms||FLJ33531, IL-1RAcPIP, 4930403G24Rik, 4931428G15Rik, fk22a02, zgc:76985, wu:fk22a02, tollip, MGC79626, TOLLIP, MGC139368, GB17961|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|4 references available|
|Quantity||400 µL (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This TOLLIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138~167 amino acids from the center region of human TOLLIP.|
|Description||Other names: Toll-interacting protein|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This TOLLIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138~167 amino acids from the center region of human TOLLIP.|
|Molecular Weight||30282 DA|
Background: The Toll/IL1R (TIR) domain is highly conserved in the cytoplasmic regions of several molecules associated with innate immunity. The deduced 274-amino acid human TOLLIP protein, which is 97% identical to the mouse sequence, features a type II C2 motif. TOLLIP binds to IL1RAP, to a complex of IL1RAP-IL1R1, and to IL18R. TOLLIP-IL1R-associated kinase-1 (IRAK1) complexes appear to exist constitutively. Furthermore, TOLLIP recruits IRAK to the IL1R complex. The TOLLIP-IRAK1 complex is terminated by stimulation with IL1B and IRAK phosphorylation. TOLLIP also interacts with TLR2 and TLR4, TOLLIP overexpression inhibits nuclear factor kappa-B (NFKB) activation in response to lipopolysaccharide and IL1B.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:50~100 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca|
|Research Area||Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cell Structure|
|Restrictions||For Research Use only|
Volpe, Clatworthy, Kaptein et al.: "The IL1 receptor accessory protein is responsible for the recruitment of the interleukin-1 receptor associated kinase to the IL1/IL1 receptor I complex." in: FEBS letters, Vol. 419, Issue 1, pp. 41-4, 1998 (PubMed).
Burns, Clatworthy, Martin et al.: "Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor." in: Nature cell biology, Vol. 2, Issue 6, pp. 346-51, 2000 (PubMed).
Bulut, Faure, Thomas et al.: "Cooperation of Toll-like receptor 2 and 6 for cellular activation by soluble tuberculosis factor and Borrelia burgdorferi outer surface protein A lipoprotein: role of Toll-interacting protein and IL-1 receptor signaling molecules in Toll-like receptor 2 s" in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 2, pp. 987-94, 2001 (PubMed).
Zhang, Ghosh: "Negative regulation of toll-like receptor-mediated signaling by Tollip." in: The Journal of biological chemistry, Vol. 277, Issue 9, pp. 7059-65, 2002 (PubMed).
|Hosts||Rabbit (48), Mouse (12), Rat (4)|
|Reactivities||Human (59), Mouse (Murine) (29), Rat (Rattus) (22), Chicken (1), Cow (Bovine) (1), Xenopus laevis (1), Zebrafish (1)|
|Applications||Western Blotting (WB) (61), ELISA (31), Immunohistochemistry (IHC) (18), Immunocytochemistry (ICC) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Immunoprecipitation (IP) (6), Immunofluorescence (IF) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Flow Cytometry (FACS) (2)|
|Epitopes||C-Term (16), N-Term (6), Internal Region (4), AA 61-274 (2), AA 1-274 (1), C-Term,AA 235-265 (1), Center (1), Middle Region (1), internal (1)|