Toll Interacting Protein (TOLLIP) (Center) antibody

Antigen: Toll Interacting Protein (TOLLIP)

Synonyms: FLJ33531, IL-1RAcPIP, 4930403G24Rik, 4931428G15Rik, fk22a02, zgc:76985, wu:fk22a02, tollip, MGC79626, TOLLIP, MGC139368, GB17961

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388941

Additional Information

Alternative name: TOLLIP

Gene ID: 54472

UniProt: NP_061882

Immunogen: This TOLLIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138~167 amino acids from the center region of human TOLLIP.

Isotype: Ig

Description: Other names: Toll-interacting protein

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This TOLLIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138~167 amino acids from the center region of human TOLLIP.

Molecular Weight: 30282 DA

Comments:

Background: The Toll/IL1R (TIR) domain is highly conserved in the cytoplasmic regions of several molecules associated with innate immunity. The deduced 274-amino acid human TOLLIP protein, which is 97% identical to the mouse sequence, features a type II C2 motif. TOLLIP binds to IL1RAP, to a complex of IL1RAP-IL1R1, and to IL18R. TOLLIP-IL1R-associated kinase-1 (IRAK1) complexes appear to exist constitutively. Furthermore, TOLLIP recruits IRAK to the IL1R complex. The TOLLIP-IRAK1 complex is terminated by stimulation with IL1B and IRAK phosphorylation. TOLLIP also interacts with TLR2 and TLR4, TOLLIP overexpression inhibits nuclear factor kappa-B (NFKB) activation in response to lipopolysaccharide and IL1B.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:50~100 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of TOLLIP (arrow) using rabbit polyclonal TOLLIP Antibody (G145) (ABIN388941). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TOLLIP gene.

TOLLIP Antibody (Center) (ABIN388941)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TOLLIP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Volpe, Clatworthy, Kaptein et al.: "The IL1 receptor accessory protein is responsible for the recruitment of the interleukin-1 receptor associated kinase to the IL1/IL1 receptor I complex." in: FEBS letters, Vol. 419, Issue 1, pp. 41-4, 1998 (PubMed).

Burns, Clatworthy, Martin et al.: "Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor." in: Nature cell biology, Vol. 2, Issue 6, pp. 346-51, 2000 (PubMed).

Bulut, Faure, Thomas et al.: "Cooperation of Toll-like receptor 2 and 6 for cellular activation by soluble tuberculosis factor and Borrelia burgdorferi outer surface protein A lipoprotein: role of Toll-interacting protein and IL-1 receptor signaling molecules in Toll-like receptor 2 s" in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 2, pp. 987-94, 2001 (PubMed).

Zhang, Ghosh: "Negative regulation of toll-like receptor-mediated signaling by Tollip." in: The Journal of biological chemistry, Vol. 277, Issue 9, pp. 7059-65, 2002 (PubMed).