Mindbomb Homolog 1 (Drosophila) (MIB1) (Center) antibody

Antigen: Mindbomb Homolog 1 (Drosophila) (MIB1)

Synonyms: MIB, ZZZ6, DIP-1, ZZANK2, FLJ90676, MGC129659, MGC129660, DKFZp686I0769, DKFZp761M1710, Mib, MGC18948, mKIAA1323, E430019M12Rik

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388958

Additional Information

Alternative name: Mib1/Mindbomb

Gene ID: 57534

UniProt: Q86YT6

Immunogen: This Mib1/Mindbomb antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 481~511 amino acids from the center region of human MIB.

Isotype: Ig

Description: Other names: E3 ubiquitin-protein ligase MIB1, Mind bomb homolog 1, DAPK-interacting protein 1, DIP-1, Zinc finger ZZ type with ankyrin repeat domain protein 2, MIB1, DIP1, KIAA1323, ZZANK2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Mib1/Mindbomb antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 481~511 amino acids from the center region of human MIB.

Molecular Weight: 110135 DA

Comments:

Background: MIB is an E3 ubiquitin ligase protein that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. It positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. It probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis

Application Details

Application Notes: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

The anti-MIB Pab (ABIN388958) is used in Western blot to detect MIB in Jurkat cell lysate.

Publications

Product: Itoh, Kim, Palardy et al.: "Mind bomb is a ubiquitin ligase that is essential for efficient activation of Notch signaling by Delta." in: Developmental cell, Vol. 4, Issue 1, pp. 67-82, 2003 (PubMed).