RAD23 Homolog B (S. Cerevisiae) (RAD23B) (N-Term) antibody

Antigen: RAD23 Homolog B (S. Cerevisiae) (RAD23B)

Synonyms: P58, HR23B, HHR23B, p58, mHR23B, AV001138, 0610007D13Rik, MGC112630, MGC137555, RAD23B, MGC107846

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388961

Additional Information

Alternative name: RAD23B

Gene ID: 5887

UniProt: P54727

Immunogen: This RAD23B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15~44 amino acids from the N-terminal region of human RAD23B.

Isotype: Ig

Description: Other names: UV excision repair protein RAD23 homolog B, HHR23B

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RAD23B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15~44 amino acids from the N-terminal region of human RAD23B.

Molecular Weight: 43171 DA

Comments:

Background: RAD23B is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

The anti-RAD23B Pab (ABIN388961) is used in Western blot to detect RAD23B in HeLa cell lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Sugasawa, Ng, Masutani et al.: "Xeroderma pigmentosum group C protein complex is the initiator of global genome nucleotide excision repair." in: Molecular cell, Vol. 2, Issue 2, pp. 223-32, 1998 (PubMed).

Hiyama, Yokoi, Masutani et al.: "Interaction of hHR23 with S5a. The ubiquitin-like domain of hHR23 mediates interaction with S5a subunit of 26 S proteasome." in: The Journal of biological chemistry, Vol. 274, Issue 39, pp. 28019-25, 1999 (PubMed).

Ng, Vermeulen, van der Horst et al.: "A novel regulation mechanism of DNA repair by damage-induced and RAD23-dependent stabilization of xeroderma pigmentosum group C protein." in: Genes & development, Vol. 17, Issue 13, pp. 1630-45, 2003 (PubMed).