SQSTM1 antibody (AA 317-346)

Catalog No. ABIN388979

Product Details anti-SQSTM1 Antibody

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Binding Specificity: AA 317-346

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SQSTM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SQSTM1 (p62) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-346 amino acids of human SQSTM1 (p62).

Clone: RB4614

Isotype: IgG

Application Details

Application Notes: IF: 1:200. IF: 1:50~100. WB: 1:2000. WB: 1:2000. IHC-P: 1:25. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-SQSTM1 antibody (ABIN388979)

Tan, Wang, Yu, Zhang, Bristow, Hill, Tannock: "Role of Autophagy as a Survival Mechanism for Hypoxic Cells in Tumors." in: Neoplasia (New York, N.Y.), Vol. 18, Issue 6, pp. 347-55, (2017) (PubMed).

Ichikawa, Alves, Pfeiffer, Langa, Hernández-Santana, Suzuki, Prehn, Engel, Henshall: "Deletion of the BH3-only protein Noxa alters electrographic seizures but does not protect against hippocampal damage after status epilepticus in mice." in: Cell death & disease, Vol. 8, Issue 1, pp. e2556, (2017) (PubMed).

Tan, Joshua, Wang, Bristow, Wouters, Allen, Tannock: "Up-regulation of autophagy is a mechanism of resistance to chemotherapy and can be inhibited by pantoprazole to increase drug sensitivity." in: Cancer chemotherapy and pharmacology, Vol. 79, Issue 5, pp. 959-969, (2017) (PubMed).

Tan, Joshua, Saggar, Yu, Wang, Kanga, Zhang, Chen, Wouters, Tannock: "Effect of pantoprazole to enhance activity of docetaxel against human tumour xenografts by inhibiting autophagy." in: British journal of cancer, Vol. 112, Issue 5, pp. 832-40, (2015) (PubMed).

Wu, Zhang, Lu, Xia, Zhou, Petrof, Claud, Chen, Chang, Carmeliet, Sun: "Intestinal epithelial vitamin D receptor deletion leads to defective autophagy in colitis." in: Gut, Vol. 64, Issue 7, pp. 1082-94, (2015) (PubMed).

Sansanwal, Li, Sarwal: "Inhibition of intracellular clusterin attenuates cell death in nephropathic cystinosis." in: Journal of the American Society of Nephrology : JASN, Vol. 26, Issue 3, pp. 612-25, (2015) (PubMed).

Zhang, Li, Wang, Chen, Dong, Nie: "Interference with HMGB1 increases the sensitivity to chemotherapy drugs by inhibiting HMGB1-mediated cell autophagy and inducing cell apoptosis." in: Tumour biology, (2015) (PubMed).

Gurpinar, Grizzle, Shacka, Mader, Li, Piazza, Russo, Keeton, Piazza: "A novel sulindac derivative inhibits lung adenocarcinoma cell growth through suppression of Akt/mTOR signaling and induction of autophagy." in: Molecular cancer therapeutics, Vol. 12, Issue 5, pp. 663-74, (2013) (PubMed).

Urtishak, Edwards, Wang, Hudome, Robinson, Barrett, Cao, Cory, Moore, Bantly, Yu, Chen, Atlas, Willman, Kundu, Carroll, Heerema, Devidas, Hilden, Dreyer, Hunger, Reaman, Felix: "Potent obatoclax cytotoxicity and activation of triple death mode killing across infant acute lymphoblastic leukemia." in: Blood, Vol. 121, Issue 14, pp. 2689-703, (2013) (PubMed).

Han, Pan, Xu, Xiao, An, Tie, Pan, Li: "Curcumin induces autophagy to protect vascular endothelial cell survival from oxidative stress damage." in: Autophagy, Vol. 8, Issue 5, pp. 812-25, (2012) (PubMed).

Soontornniyomkij, Risbrough, Young, Soontornniyomkij, Jeste, Achim: "Increased hippocampal accumulation of autophagosomes predicts short-term recognition memory impairment in aged mice." in: Age (Dordrecht, Netherlands), Vol. 34, Issue 2, pp. 305-16, (2012) (PubMed).

Lee, Shin, Yuk, Shi, Choi, Lee, Huang, Kim, Kim, Lee, Jo: "Autophagy negatively regulates keratinocyte inflammatory responses via scaffolding protein p62/SQSTM1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 2, pp. 1248-58, (2011) (PubMed).

Okamoto, Sakimoto, Imai, Senoo, Shidoji: "Induction of an incomplete autophagic response by cancer-preventive geranylgeranoic acid (GGA) in a human hepatoma-derived cell line." in: The Biochemical journal, Vol. 440, Issue 1, pp. 63-71, (2011) (PubMed).

Nascimento-Ferreira, Santos-Ferreira, Sousa-Ferreira, Auregan, Onofre, Alves, Dufour, Colomer Gould, Koeppen, Déglon, Pereira de Almeida: "Overexpression of the autophagic beclin-1 protein clears mutant ataxin-3 and alleviates Machado-Joseph disease." in: Brain : a journal of neurology, Vol. 134, Issue Pt 5, pp. 1400-15, (2011) (PubMed).

Zhang, Lowry, Guarente, Haimovich: "Roles of SIRT1 in the acute and restorative phases following induction of inflammation." in: The Journal of biological chemistry, Vol. 285, Issue 53, pp. 41391-401, (2010) (PubMed).

Maynard, Dvorak, Khailova, Dobrenen, Arganbright, Halpern, Kurundkar, Maheshwari, Dvorak: "Epidermal growth factor reduces autophagy in intestinal epithelium and in the rat model of necrotizing enterocolitis." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 299, Issue 3, pp. G614-22, (2010) (PubMed).

Lin, Whang, Donner, Du, Lorch, He, Jiang, Price, Moore, Ruan: "Autophagy induction with RAD001 enhances chemosensitivity and radiosensitivity through Met inhibition in papillary thyroid cancer." in: Molecular cancer research : MCR, Vol. 8, Issue 9, pp. 1217-26, (2010) (PubMed).

Chu, Wang, Wu, Lei: "Invasion and multiplication of Helicobacter pylori in gastric epithelial cells and implications for antibiotic resistance." in: Infection and immunity, Vol. 78, Issue 10, pp. 4157-65, (2010) (PubMed).

Wang, Gorvel, Chu, Wu, Lei: "Helicobacter pylori impairs murine dendritic cell responses to infection." in: PLoS ONE, Vol. 5, Issue 5, pp. e10844, (2010) (PubMed).

Kobayashi, Volden, Timm, Mao, Xu, Liang: "Transcription factor GATA4 inhibits doxorubicin-induced autophagy and cardiomyocyte death." in: The Journal of biological chemistry, Vol. 285, Issue 1, pp. 793-804, (2009) (PubMed).

Target Details for SQSTM1

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Alternative Name: SQSTM1 (p62) (SQSTM1 Products)

Synonyms: SQSTM1 antibody, sqstm1 antibody, MGC79491 antibody, A170 antibody, OSIL antibody, PDB3 antibody, ZIP3 antibody, p60 antibody, p62 antibody, p62B antibody, OSF-6 antibody, Osi antibody, STAP antibody, ZIP antibody, sb:cb621 antibody, zgc:85784 antibody, sequestosome 1 antibody, sequestosome 1 L homeolog antibody, SQSTM1 antibody, sqstm1 antibody, Sqstm1 antibody, sqstm1.L antibody

Background: SQSTM1/p62 is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. This protein may play a role in titin/TTN downstream signaling in muscle cells, and may also regulate signaling cascades through ubiquitination. This protein is involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. SQSTM1/p62 also appears to play a role in macroautophagic removal of intracellular protein aggregates. Cellular depletion studies of SQSTM1/p62 have indicated a role for association with LC3 and aggregate proteins in order to facilitate normal formation of the autophagosome.

Molecular Weight: 47687

Gene ID: 8878

NCBI Accession: NP_001135770, NP_001135771, NP_003891

UniProt: Q13501

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy