SQSTM1 antibody (Sequestosome 1) (AA 317-346)

Details for Product anti-SQSTM1 Antibody No. ABIN388979, Supplier: Log in to see
Antigen
  • SQSTM1
  • sqstm1
  • MGC79491
  • A170
  • OSIL
  • PDB3
  • ZIP3
  • p60
  • p62
  • p62B
  • OSF-6
  • Osi
  • STAP
  • ZIP
  • sb:cb621
  • zgc:85784
  • sequestosome 1
  • sqstm1 protein
  • SQSTM1
  • LOC100342157
  • sqstm1
  • Sqstm1
Alternatives
anti-Human SQSTM1 antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 317-346, C-Term
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40
22
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5
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2
2
2
1
1
1
1
1
1
1
1
1
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1
Reactivity
Human
331
152
101
8
5
5
5
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4
2
2
2
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2
1
1
1
1
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1
Host
Rabbit
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68
4
3
Clonality (Clone)
Polyclonal ()
Conjugate
This SQSTM1 antibody is un-conjugated
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1
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Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
264
137
96
85
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64
52
35
33
14
13
4
4
3
2
Options
Supplier
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Supplier Product No.
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Immunogen This SQSTM1 (p62) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-346 AA from the C-terminal region of human SQSTM1 (p62).
Clone RB4614
Isotype Ig
Predicted Reactivity Mouse (Murine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name SQSTM1 (p62) (SQSTM1 Antibody Abstract)
Background SQSTM1/p62 is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. This protein may play a role in titin/TTN downstream signaling in muscle cells, and may also regulate signaling cascades through ubiquitination. This protein is involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. SQSTM1/p62 also appears to play a role in macroautophagic removal of intracellular protein aggregates. Cellular depletion studies of SQSTM1/p62 have indicated a role for association with LC3 and aggregate proteins in order to facilitate normal formation of the autophagosome.

Synonyms: Sequestosome-1, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, EBI3-associated protein of 60 kDa, p60, EBIAP, SQSTM1, ORCA, OSIL
Pathways: Osteoclast differentiation
Molecular Weight 47687 Da
Gene ID 8878
UniProt Q13501
Research Area Ubiquitin-related antibodies, Apoptosis/Necrosis, Cell Signaling, Protein Modifications, Cell Cycle, Autophagy, Cell Structure
Pathways NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy
Application Notes Suggested dilutions:
IF = 1:50-100, WB = 1:1000, IHC (p) = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Sequestosome 1 (SQSTM1) (AA 317-346), (C-Term) antibody (ABIN388979) Western blot analysis of hSQSTM1-C331 (ABIN388979) in A2058 cell line lysates (35 µg/...
Western Blotting (WB) image for anti-Sequestosome 1 (SQSTM1) (AA 317-346), (C-Term) antibody (ABIN388979) Western blot analysis of SQSTM1 (arrow) using rabbit polyclonal Autophagy SQSTM1 (p62...
Immunohistochemistry (IHC) image for anti-Sequestosome 1 (SQSTM1) (AA 317-346), (C-Term) antibody (ABIN388979) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Product cited in: Tan, Joshua, Saggar, Yu, Wang, Kanga, Zhang, Chen, Wouters, Tannock: "Effect of pantoprazole to enhance activity of docetaxel against human tumour xenografts by inhibiting autophagy." in: British journal of cancer, Vol. 112, Issue 5, pp. 832-40, 2015 (PubMed).

Wu, Zhang, Lu, Xia, Zhou, Petrof, Claud, Chen, Chang, Carmeliet, Sun: "Intestinal epithelial vitamin D receptor deletion leads to defective autophagy in colitis." in: Gut, Vol. 64, Issue 7, pp. 1082-94, 2015 (PubMed).

Sansanwal, Li, Sarwal: "Inhibition of intracellular clusterin attenuates cell death in nephropathic cystinosis." in: Journal of the American Society of Nephrology : JASN, Vol. 26, Issue 3, pp. 612-25, 2015 (PubMed).

Zhang, Li, Wang, Chen, Dong, Nie: "Interference with HMGB1 increases the sensitivity to chemotherapy drugs by inhibiting HMGB1-mediated cell autophagy and inducing cell apoptosis." in: Tumour biology, 2015 (PubMed).

Gurpinar, Grizzle, Shacka, Mader, Li, Piazza, Russo, Keeton, Piazza: "A novel sulindac derivative inhibits lung adenocarcinoma cell growth through suppression of Akt/mTOR signaling and induction of autophagy." in: Molecular cancer therapeutics, Vol. 12, Issue 5, pp. 663-74, 2013 (PubMed).

Urtishak, Edwards, Wang, Hudome, Robinson, Barrett, Cao, Cory, Moore, Bantly, Yu, Chen, Atlas, Willman, Kundu, Carroll, Heerema, Devidas, Hilden, Dreyer, Hunger, Reaman, Felix: "Potent obatoclax cytotoxicity and activation of triple death mode killing across infant acute lymphoblastic leukemia." in: Blood, Vol. 121, Issue 14, pp. 2689-703, 2013 (PubMed).

Han, Pan, Xu, Xiao, An, Tie, Pan, Li: "Curcumin induces autophagy to protect vascular endothelial cell survival from oxidative stress damage." in: Autophagy, Vol. 8, Issue 5, pp. 812-25, 2012 (PubMed).

Soontornniyomkij, Risbrough, Young, Soontornniyomkij, Jeste, Achim: "Increased hippocampal accumulation of autophagosomes predicts short-term recognition memory impairment in aged mice." in: Age (Dordrecht, Netherlands), Vol. 34, Issue 2, pp. 305-16, 2012 (PubMed).

Lee, Shin, Yuk, Shi, Choi, Lee, Huang, Kim, Kim, Lee, Jo: "Autophagy negatively regulates keratinocyte inflammatory responses via scaffolding protein p62/SQSTM1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 2, pp. 1248-58, 2011 (PubMed).

Okamoto, Sakimoto, Imai, Senoo, Shidoji: "Induction of an incomplete autophagic response by cancer-preventive geranylgeranoic acid (GGA) in a human hepatoma-derived cell line." in: The Biochemical journal, Vol. 440, Issue 1, pp. 63-71, 2011 (PubMed).

Nascimento-Ferreira, Santos-Ferreira, Sousa-Ferreira, Auregan, Onofre, Alves, Dufour, Colomer Gould, Koeppen, Déglon, Pereira de Almeida: "Overexpression of the autophagic beclin-1 protein clears mutant ataxin-3 and alleviates Machado-Joseph disease." in: Brain : a journal of neurology, Vol. 134, Issue Pt 5, pp. 1400-15, 2011 (PubMed).

Zhang, Lowry, Guarente, Haimovich: "Roles of SIRT1 in the acute and restorative phases following induction of inflammation." in: The Journal of biological chemistry, Vol. 285, Issue 53, pp. 41391-401, 2010 (PubMed).

Maynard, Dvorak, Khailova, Dobrenen, Arganbright, Halpern, Kurundkar, Maheshwari, Dvorak: "Epidermal growth factor reduces autophagy in intestinal epithelium and in the rat model of necrotizing enterocolitis." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 299, Issue 3, pp. G614-22, 2010 (PubMed).

Lin, Whang, Donner, Du, Lorch, He, Jiang, Price, Moore, Ruan: "Autophagy induction with RAD001 enhances chemosensitivity and radiosensitivity through Met inhibition in papillary thyroid cancer." in: Molecular cancer research : MCR, Vol. 8, Issue 9, pp. 1217-26, 2010 (PubMed).

Chu, Wang, Wu, Lei: "Invasion and multiplication of Helicobacter pylori in gastric epithelial cells and implications for antibiotic resistance." in: Infection and immunity, Vol. 78, Issue 10, pp. 4157-65, 2010 (PubMed).

Wang, Gorvel, Chu, Wu, Lei: "Helicobacter pylori impairs murine dendritic cell responses to infection." in: PLoS ONE, Vol. 5, Issue 5, pp. e10844, 2010 (PubMed).

Kobayashi, Volden, Timm, Mao, Xu, Liang: "Transcription factor GATA4 inhibits doxorubicin-induced autophagy and cardiomyocyte death." in: The Journal of biological chemistry, Vol. 285, Issue 1, pp. 793-804, 2009 (PubMed).

Tal, Sasai, Lee, Yordy, Shadel, Iwasaki: "Absence of autophagy results in reactive oxygen species-dependent amplification of RLR signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 8, pp. 2770-5, 2009 (PubMed).

Ju, Miller, Hanson, Weihl: "Impaired protein aggregate handling and clearance underlie the pathogenesis of p97/VCP-associated disease." in: The Journal of biological chemistry, Vol. 283, Issue 44, pp. 30289-99, 2008 (PubMed).

Background publications Seibenhener, Babu, Geetha, Wong, Krishna, Wooten: "Sequestosome 1/p62 is a polyubiquitin chain binding protein involved in ubiquitin proteasome degradation." in: Molecular and cellular biology, Vol. 24, Issue 18, pp. 8055-68, 2004 (PubMed).

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