Lethal Giant Larvae Homolog 1 (Drosophila) (LLGL1) (C-Term) antibody

Antigen: Lethal Giant Larvae Homolog 1 (Drosophila) (LLGL1)

Synonyms: DLG4, HUGL, LLGL, HUGL1, HUGL-1, Mgl1, Llglh, AI325176, Rgl1

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388989

Additional Information

Alternative name: LLGL1

Gene ID: 3996

UniProt: Q15334

Immunogen: This LLGL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human LLGL1.

Isotype: Ig

Description: Other names: Hugl protein, LLGL1, hugl,

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This LLGL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human LLGL1.

Molecular Weight: 114379 DA

Comments:

Background: LLGL1 is a protein that is similar to a tumor suppressor in Drosophila. The protein is part of a cytoskeletal network and is associated with nonmuscle myosin II heavy chain and a kinase that specifically phosphorylates this protein at serine residues. The gene for LLGL1 is located within the Smith-Magenis syndrome region on chromosome 17.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western Blot~~1:250~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Signaling, Cell Structure

Restrictions: For Research Use only

Images

The anti-LLGL1 Pab (A2197b) is used in Western blot to detect LLGL1 in HepG2 cell line lysate. Blocking peptide (BP2197b) was incubated with anti-LLGL1 Pab (A2197b) in the right lane.

Publications

Product: Katoh, Katoh: "Identification and characterization of human LLGL4 gene and mouse Llgl4 gene in silico." in: International journal of oncology, Vol. 24, Issue 3, pp. 737-42, 2004 (PubMed).

Grifoni, Garoia, Schimanski et al.: "The human protein Hugl-1 substitutes for Drosophila lethal giant larvae tumour suppressor function in vivo." in: Oncogene, Vol. 23, Issue 53, pp. 8688-94, 2004 (PubMed).

Schimanski, Schmitz, Kashyap et al.: "Reduced expression of Hugl-1, the human homologue of Drosophila tumour suppressor gene lgl, contributes to progression of colorectal cancer." in: Oncogene, Vol. 24, Issue 19, pp. 3100-9, 2005 (PubMed).