ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3) (N-Term) antibody

Antigen: ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3)

Synonyms: ARFGAP3, xgap, arfgap1, MGC53588, MGC145008, ARFGAP1, GAP, PKWS, RASA, CMAVM, CM-AVM, RASGAP, p120GAP, p120RASGAP, DKFZp434N071

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388995

Additional Information

Alternative name: ARFGAP3

Gene ID: 26286

UniProt: NP_055385

Immunogen: This ARFGAP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2~31 amino acids from the N-terminal region of human ARFGAP3.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ARFGAP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2~31 amino acids from the N-terminal region of human ARFGAP3.

Molecular Weight: 56928 DA

Comments:

Background: ARFGAP3 is an GTPase-activating protein (GAP) probably for ARF1. This protein may be involved in protein secretion and/or vesicle transport. It likely participates in promoting hydrolysis of the ARF1-bound GTP and thus, may be required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Like other ARFGAPs, its activity is sensitive to phospholipids. Location is cytoplasmic, concentrated in the perinuclear region. Highest expression occurs in the endocrine glands (pancreas, pituary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary. ARFGAP3 is expressed at higher level in adult thymus, brain and lung, than in corresponding fetal tissues. It is expressed at lower level in spleen, heart, kidney and liver during development.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western Blot ~~ 1:50~200 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ARFGAP3 (arrow) using rabbit polyclonal ARFGAP3 Antibody (N-term) (ABIN388995). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ARFGAP3 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Dunham, Shimizu, Roe et al.: "The DNA sequence of human chromosome 22." in: Nature, Vol. 402, Issue 6761, pp. 489-95, 1999 (PubMed).

Zhang, Yu, Zhang et al.: "Characterization, chromosomal assignment, and tissue expression of a novel human gene belonging to the ARF GAP family." in: Genomics, Vol. 63, Issue 3, pp. 400-8, 2000 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).