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Antibodies
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anti-HSF2 (Sumoylation Site) antibody
HSF2 (Sumoylation Site) antibody
| Antigen |
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Binding Site
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (47), Mouse (Murine) (39), Rat (Rattus) (32), Cow (Bovine) (5), Dog (Canine) (4), Monkey (4), Guinea Pig (3), Hamster (3), Pig (Porcine) (3), Rabbit (3), Sheep (Ovine) (3), Various Species (2), Cat (Feline) (1), Chicken (1)
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Conjugate
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Western Blotting (WB) (35), ELISA (23), Immunofluorescence (IF) (21), Immunohistochemistry (IHC) (8), Immunoprecipitation (IP) (8), Immunocytochemistry (ICC) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Electrophoretic Mobility-Shift Assay (EMSA) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Dot Blot (Dot) (1), Flow Cytometry (FACS) (1), Gel Shift (GS) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN389070 |
| Quantity |
400 µL (0.25 mg/ml) |
| Price |
280.50 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Will be delivered in 2 to 3 Business Days |
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UniProt
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Q03933
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Immunogen
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This antibody is generated from rabbits immunized with the KLH conjugated synthetic peptide VHIDSGIVKQERDGP containing a predicted sumoylation site from the N-terminal region of human HSF2.
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Isotype
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Ig
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Description
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Other names: Heat shock factor protein 2, HSF 2, Heat shock transcription factor 2, HSTF 2, HSTF2
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Characteristics
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Purified Rabbit Polyclonal Antibody (Pab)
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Specificity
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This HSF2 Sumoylation Site antibody is generated from rabbits immunized with the KLH conjugated synthetic peptide VHIDSGIVKQERDGP containing a predicted sumoylation site from the N-terminal region of human HSF2.
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Comments
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Background: HSF2 is a DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. The protein is found as a DNA-binding homotrimer in stressed or heat shocked cells, and otherwise found as a homodimer. HSF2 is cytoplasmic during normal growth and moves to the nucleus upon activation. Sumoylation of HSF2 hinders HSF2 DNA-binding activity, without affecting its oligomerization, and is an example of negative regulation of gene expression via sumoylation.
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Application Notes
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The suggested dilution is: ELISA 1:1,000 Western blotting 1:100~500 Immunohistochemistry 1:10~50
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Concentration
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0.25 mg/ml
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Purification
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Purified
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Buffer
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Research Area
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Apoptosis/Necrosis, Cell Signaling, Protein Modifications, Transcription Factors, Signaling, Cell Structure
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Restrictions
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For Research Use only
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Product
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Schuetz, Gallo, Sheldon et al.: "Isolation of a cDNA for HSF2: evidence for two heat shock factor genes in humans." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 16, pp. 6911-5, 1991 (PubMed).
He, Soncin, Grammatikakis et al.: "Elevated expression of heat shock factor (HSF) 2A stimulates HSF1-induced transcription during stress." in: The Journal of biological chemistry, Vol. 278, Issue 37, pp. 35465-75, 2003 (PubMed).
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Alternatives for antigen "HSF2", type "Antibodies"
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Hosts
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Rabbit (42), Rat (11), Mouse (3)
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Reactivities
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Human (47), Mouse (Murine) (39), Rat (Rattus) (32), Cow (Bovine) (5), Dog (Canine) (4), Monkey (4), Guinea Pig (3), Hamster (3), Pig (Porcine) (3), Rabbit (3), Sheep (Ovine) (3), Various Species (2), Cat (Feline) (1), Chicken (1)
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Applications
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Western Blotting (WB) (35), ELISA (23), Immunofluorescence (IF) (21), Immunohistochemistry (IHC) (8), Immunoprecipitation (IP) (8), Immunocytochemistry (ICC) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Electrophoretic Mobility-Shift Assay (EMSA) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Dot Blot (Dot) (1), Flow Cytometry (FACS) (1), Gel Shift (GS) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Epitopes
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C-Term (24), N-Term (3), Internal Region (1), Internal Region,AA 215-245 (1), Sumoylation Site (1)
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