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SUMO1 Activating Enzyme Subunit 1 (SAE1) (N-Term) antibody
|Synonyms||AOS1, SUA1, UBLE1A, FLJ3091, HSPC140|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|3 references available|
|Quantity||400 µL (0.25 mg/ml) (Variants)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Alternative name||SAE1 (AOS1)|
|Immunogen||This SAE1 (AOS1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8~37 amino acids from the N-terminal region of human AOS1.|
|Description||Other names: SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A, SAE1, AOS1, SUA1, UBLE1A|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This SAE1 (AOS1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8~37 amino acids from the N-terminal region of human AOS1.|
|Molecular Weight||38450 DA|
Background: The dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Apoptosis/Necrosis, Cell Signaling, Protein Modifications, Cell Structure|
|Restrictions||For Research Use only|
Okuma, Honda, Ichikawa et al.: "In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2." in: Biochemical and biophysical research communications, Vol. 254, Issue 3, pp. 693-8, 1999 (PubMed).
Desterro, Rodriguez, Kemp et al.: "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10618-24, 1999 (PubMed).
Gong, Li, Millas et al.: "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex." in: FEBS letters, Vol. 448, Issue 1, pp. 185-9, 1999 (PubMed).
|Hosts||Rabbit (49), Mouse (5), Goat (4), Sheep (1)|
|Reactivities||Human (51), Rat (Rattus) (11), Mouse (Murine) (10), Cow (Bovine) (9), Dog (Canine) (5)|
|Applications||Western Blotting (WB) (54), ELISA (45), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Immunofluorescence (IF) (7), Immunohistochemistry (IHC) (7), Fluorescence Microscopy (FM) (3), Immunocytochemistry (ICC) (2), Dot Blot (Dot) (1)|
|Epitopes||C-Term (13), N-Term (7), pSer185 (5), Internal Region (4)|