anti-SAE1 antibody (SUMO1 Activating Enzyme Subunit 1) (AA 300-329)

Details for Product anti-SAE1 Antibody No. ABIN389081
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms AOS1, CDGAP, HSPC140, SUA1, UBLE1A, 2400010M20Rik, 2610044L12Rik, AL033372, AW743391, D7Ertd177e, Sua1, Uble1a, Aos1p, Sua1p, aos
Epitope
AA 300-329, C-Term
(37), (6), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(114), (32), (30), (9), (8), (3), (1), (1), (1)
Host
Rabbit
(84), (16), (15), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
This SAE1 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(105), (70), (35), (21), (16), (9), (5), (3), (3), (3), (2), (2)
Pubmed 3 references available
Quantity 400 μL
Options
Shipping to United States ( )
Availability Will be delivered in 2 to 4 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Immunogen This SAE1 (AOS1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-329 AA from the C-terminal region of human SAE1 (AOS1).
Clone RB2887-RB2888
Isotype Ig
Specificity This SAE1 (AOS1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 307~337 amino acids from the C-terminal region of human AOS1.
Predicted Reactivity Cow (Bovine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name SAE1 (AOS1) (SAE1 Antibody Abstract)
Background The dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.
Synonyms: SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A, SAE1, AOS1, SUA1, UBLE1A
Molecular Weight 38450 DA
Gene ID 10055
UniProt Q9UBE0
Research Area Apoptosis/Necrosis, Cell Signaling, Protein Modifications, Cell Structure
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 300-329), (C-Term) antibody The AOS1 C-term Pab (ABIN389081) is used in Western blot to detect AOS1 in mouse hear...
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 300-329), (C-Term) antibody (2) Western blot analysis of AOS1 (arrow) using rabbit polyclonal AOS1 Antibody (C-term) ...
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 300-329), (C-Term) antibody (3) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Background publications Gong, Li, Millas et al.: "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex." in: FEBS letters, Vol. 448, Issue 1, pp. 185-9, 1999 (PubMed).

Desterro, Rodriguez, Kemp et al.: "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10618-24, 1999 (PubMed).

Okuma, Honda, Ichikawa et al.: "In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2." in: Biochemical and biophysical research communications, Vol. 254, Issue 3, pp. 693-8, 1999 (PubMed).

Validation Images
Catalog No. ABIN389081
291.50 $
Plus shipping costs $45.00
Quantity
Price
400 μL
291.50 $

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)
Did you look for something else?