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SAE1 antibody (SUMO1 Activating Enzyme Subunit 1) (AA 300-329)

Details for Product anti-SAE1 Antibody No. ABIN389081, Supplier: Log in to see
Antigen
  • 2400010M20Rik
  • 2610044L12Rik
  • AL033372
  • aos
  • AOS1
  • Aos1p
  • AW743391
  • CDGAP
  • D7Ertd177e
  • HSPC140
  • Sua1
  • SUA1
  • Sua1p
  • UBLE1A
  • Uble1a
Epitope
AA 300-329, C-Term
38
8
7
6
4
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
125
31
27
9
8
2
2
1
1
1
Host
Rabbit
82
27
15
1
Clonality (Clone)
Polyclonal ()
Conjugate
This SAE1 antibody is un-conjugated
3
3
3
3
3
3
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
109
65
35
27
20
10
4
4
4
1
1
1
1
Supplier
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Immunogen This SAE1 (AOS1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-329 AA from the C-terminal region of human SAE1 (AOS1).
Clone RB2887-RB2888
Isotype Ig
Specificity This SAE1 (AOS1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 307~337 amino acids from the C-terminal region of human AOS1.
Predicted Reactivity Cow (Bovine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name SAE1 (AOS1) (SAE1 Antibody Abstract)
Background The dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.
Synonyms: SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A, SAE1, AOS1, SUA1, UBLE1A
Molecular Weight 38450 DA
Gene ID 10055
UniProt Q9UBE0
Research Area Apoptosis/Necrosis, Cell Signaling, Protein Modifications, Cell Structure
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-SAE1 antibody (SUMO1 Activating Enzyme Subunit 1) (AA 300-329) (ABIN389081) The AOS1 C-term Pab (ABIN389081) is used in Western blot to detect AOS1 in mouse hear...
Western Blotting (WB) image for anti-SAE1 antibody (SUMO1 Activating Enzyme Subunit 1) (AA 300-329) (ABIN389081) Western blot analysis of AOS1 (arrow) using rabbit polyclonal AOS1 Antibody (C-term) ...
Immunohistochemistry (IHC) image for anti-SAE1 antibody (SUMO1 Activating Enzyme Subunit 1) (AA 300-329) (ABIN389081) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Background publications Desterro, Rodriguez, Kemp, Hay: "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10618-24, 1999

Gong, Li, Millas, Yeh: "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex." in: FEBS letters, Vol. 448, Issue 1, pp. 185-9, 1999

Okuma, Honda, Ichikawa, Tsumagari, Yasuda: "In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2." in: Biochemical and biophysical research communications, Vol. 254, Issue 3, pp. 693-8, 1999