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SUMO1 Activating Enzyme Subunit 1 (SAE1) (C-Term), (AA 300-329) antibody

Details for Product No. ABIN389081
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Antigen
Synonyms AOS1, HSPC140, SUA1, UBLE1A, 2400010M20Rik, 2610044L12Rik, AL033372, AW743391, D7Ertd177e, Sua1, Uble1a, Aos1p, Sua1p, aos
Epitope
C-Term, AA 300-329
(20), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(85), (26), (24), (8), (7), (3)
Host
Rabbit
(71), (11), (6), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(80), (58), (27), (22), (10), (9), (3), (3), (2), (1)
Pubmed 3 references available
Quantity 400 µL
Options
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Catalog No. ABIN389081
291.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This SAE1 (AOS1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-329 AA from the C-terminal region of human SAE1 (AOS1).
Clone RB2887-RB2888
Isotype Ig
Specificity This SAE1 (AOS1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 307~337 amino acids from the C-terminal region of human AOS1.
Predicted Reactivity Cow (Bovine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name SAE1 (AOS1)
Background The dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.
Synonyms: SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A, SAE1, AOS1, SUA1, UBLE1A
Molecular Weight 38450 DA
Gene ID 10055
UniProt Q9UBE0
Research Area Apoptosis/Necrosis, Cell Signaling, Protein Modifications, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (C-Term), (AA 300-329) antibody The AOS1 C-term Pab (ABIN389081) is used in Western blot to detect AOS1 in mouse heart tissue lysate.
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (C-Term), (AA 300-329) antibody (2) Western blot analysis of AOS1 (arrow) using rabbit polyclonal AOS1 Antibody (C-term) (ABIN389081). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the AOS1 gene (Lane 2) (Origene Technologies).
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (C-Term), (AA 300-329) antibody (3) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Okuma, Honda, Ichikawa et al.: "In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2." in: Biochemical and biophysical research communications, Vol. 254, Issue 3, pp. 693-8, 1999 (PubMed).

Desterro, Rodriguez, Kemp et al.: "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10618-24, 1999 (PubMed).

Gong, Li, Millas et al.: "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex." in: FEBS letters, Vol. 448, Issue 1, pp. 185-9, 1999 (PubMed).

Hosts (71), (11), (6), (1)
Reactivities (85), (26), (24), (8), (7), (3)
Applications (80), (58), (27), (22), (10), (9), (3), (3), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1)
Epitopes (20), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN389081
291.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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