Chromobox Homolog 8 (CBX8) (N-Term) antibody

Antigen: Chromobox Homolog 8 (CBX8)

Synonyms: PC3, RC1, HPC3, cbx8, MGC147589

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389088

Additional Information

Alternative name: CBX8

Gene ID: 57332

UniProt: Q9HC52

Immunogen: This CBX8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91~120 amino acids from the N-terminal region of human CBX8.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CBX8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91~120 amino acids from the N-terminal region of human CBX8.

Molecular Weight: 43437 DA

Comments:

Background: Chromobox homolog 8 (CBX8 or Pc3) is a member of Drosophila Polycomb group gene family. The polycomb group (PcG) genes are essential for maintenance of appropriate expression patterns of developmental master regulators and thus are essential for proper development. Changes in expression of PcG proteins have been associated with cancer. CBX8 is involved in maintaining the transcriptionally repressive state of genes. It modifies chromatin, rendering it heritably changed in its expressibility. Structurally, CBX4 contains 1 chromo domain, which is 40 to 50 amino acids long.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Protein Modifications, Cancer, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

The anti-CBX8 N-term Pab (ABIN389088) is used in Western blot to detect CBX8 in Jurkat cell lysate.

Publications

Product: Bárdos, Saurin, Tissot et al.: "HPC3 is a new human polycomb orthologue that interacts and associates with RING1 and Bmi1 and has transcriptional repression properties." in: The Journal of biological chemistry, Vol. 275, Issue 37, pp. 28785-92, 2000 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).