Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 689-719), (Loop 2) antibody

Details for Product No. ABIN389218
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Antigen
Synonyms lgr5-a, lgr5a, FEX, Gpr49, GPR49, GPR67, GRP49, HG38
Epitope
AA 689-719, Loop 2
(7), (6), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(77), (28), (19), (17), (17), (17)
Host
Rabbit
(65), (14)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(48), (25), (21), (16), (16), (14), (11), (11), (1), (1)
Pubmed 1 reference available
Quantity 400 μL
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Catalog No. ABIN389218
291.50 $
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Immunogen This LGR5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 689-719 AA from human LGR5.
Clone RB14211
Isotype Ig
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name LGR5/GPR49
Background Function:
Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R- spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.

Cellular Location:
Cell membrane, Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane, Multi-pass membrane protein. Note=Rapidly and constitutively internalized to the trans-Golgi network at steady state. Internalization to the trans- Golgi network may be the result of phosphorylation at Ser-861 and Ser-864, however, the phosphorylation event has not been proven

Tissue Location:
Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver

Synonyms: Leucine-rich repeat-containing G-protein coupled receptor 5 [Precursor], Orphan G-protein coupled receptor HG38, G-protein coupled receptor 49, G-protein coupled receptor 67, LGR5, GPR49, GPR67
Molecular Weight 99867 Da
Gene ID 8549
UniProt O75473
Research Area Stem Cells, Cell Signaling, Cell Structure
Application Notes Suggested dilutions:
IF = 1:100, WB = 1:1000, IHC = 1:10-50, FACS = 1:10-50
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 689-719), (Loop 2) antibody LGR5/GPR49 Antibody (Loop2) (ABIN389218) western blot analysis in HepG2 cell line lysates (35 µg/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow)(Kindly offered by Dr. Li).
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 689-719), (Loop 2) antibody (2) Western blot analysis of anti-LGR5/GPR49 Antibody (loop2) (ABIN389218) in mouse cerebellum tissue lysates (35 µg/lane).LGR5/GPR49(arrow) was detected using the purified polyclonal antibody.
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 689-719), (Loop 2) antibody (3) Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (loop2) (ABIN389218), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Background publications Barker, van Es, Kuipers et al.: "Identification of stem cells in small intestine and colon by marker gene Lgr5." in: Nature, Vol. 449, Issue 7165, pp. 1003-7, 2007 (PubMed).

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