anti-LGR5 antibody (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5) (AA 689-719)

Details for Product anti-LGR5 Antibody No. ABIN389218
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Antigen
Synonyms lgr5-a, lgr5a, FEX, Gpr49, GPR49, GPR67, GRP49, HG38
Epitope
AA 689-719, Loop 2
(21), (8), (6), (6), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(94), (20), (3)
Host
Rabbit
(61), (35)
Clonality (Clone)
Polyclonal ()
Conjugate
This LGR5 antibody is un-conjugated
(1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(60), (35), (23), (21), (20), (14), (10), (3), (3), (1), (1), (1)
Pubmed 7 references available
Quantity 400 μL
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Immunogen This LGR5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 689-719 AA from human LGR5.
Clone RB14211
Isotype Ig
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name LGR5/GPR49 (LGR5 Antibody Abstract)
Background Function:
Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R- spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.

Cellular Location:
Cell membrane, Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane, Multi-pass membrane protein. Note=Rapidly and constitutively internalized to the trans-Golgi network at steady state. Internalization to the trans- Golgi network may be the result of phosphorylation at Ser-861 and Ser-864, however, the phosphorylation event has not been proven

Tissue Location:
Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver

Synonyms: Leucine-rich repeat-containing G-protein coupled receptor 5 [Precursor], Orphan G-protein coupled receptor HG38, G-protein coupled receptor 49, G-protein coupled receptor 67, LGR5, GPR49, GPR67
Molecular Weight 99867 Da
Gene ID 8549
UniProt O75473
Research Area Stem Cells, Cell Signaling, Cell Structure
Application Notes Suggested dilutions:
IF = 1:100, WB = 1:1000, IHC (p) = 1:10-50, FACS = 1:10-50
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 689-719), (Loop 2) antibody LGR5/GPR49 Antibody (Loop2) (ABIN389218) western blot analysis in HepG2 cell line lys...
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 689-719), (Loop 2) antibody (2) Western blot analysis of anti-LGR5/GPR49 Antibody (loop2) (ABIN389218) in mouse cereb...
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 689-719), (Loop 2) antibody (3) Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antib...
Product cited in: Huch, Gehart, van Boxtel et al.: "Long-term culture of genome-stable bipotent stem cells from adult human liver. ..." in: Cell, Vol. 160, Issue 1-2, pp. 299-312, 2015 (PubMed).

Zheng, Sun, Bu et al.: "Intestinal stem cell marker LGR5 expression during gastric carcinogenesis." in: World journal of gastroenterology : WJG, Vol. 19, Issue 46, pp. 8714-21, 2013 (PubMed).

Bu, Zheng, Zhang et al.: "LGR5 is a promising biomarker for patients with stage I and II gastric cancer." in: Chinese journal of cancer research = Chung-kuo yen cheng yen chiu, Vol. 25, Issue 1, pp. 79-89, 2013 (PubMed).

Maddox, Shakya, South et al.: "Transcription factor Oct1 is a somatic and cancer stem cell determinant." in: PLoS genetics, Vol. 8, Issue 11, pp. e1003048, 2012 (PubMed).

Takahashi, Ishii, Nishida et al.: "Significance of Lgr5(+ve) cancer stem cells in the colon and rectum." in: Annals of surgical oncology, Vol. 18, Issue 4, pp. 1166-74, 2011 (PubMed).

Satoh, Obayashi, Tabunoki et al.: "Stable expression of neurogenin 1 induces LGR5, a novel stem cell marker, in an immortalized human neural stem cell line HB1.F3." in: Cellular and molecular neurobiology, Vol. 30, Issue 3, pp. 415-26, 2010 (PubMed).

Background publications Barker, van Es, Kuipers et al.: "Identification of stem cells in small intestine and colon by marker gene Lgr5." in: Nature, Vol. 449, Issue 7165, pp. 1003-7, 2007 (PubMed).

Validation Images
Catalog No. ABIN389218
291.50 $
Plus shipping costs $45.00
Quantity
Price
400 μL
291.50 $

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