Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (Loop 2) antibody

Antigen: Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5)

Synonyms: FEX, HG38, GPR49, GPR67, GRP49, MGC117008, lgr5a

Binding Site: Loop 2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389218

Additional Information

Alternative name: LGR5/GPR49

Gene ID: 8549

UniProt: O75473

Immunogen: This LGR5/GPR49 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 697~726 amino acids from the loop2 region of human LGR5/GPR49.

Isotype: Ig

Description: Other names: Leucine-rich repeat-containing G-protein coupled receptor 5 [Precursor], Orphan G-protein coupled receptor HG38, G-protein coupled receptor 49, G-protein coupled receptor 67, LGR5, GPR49, GPR67

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This LGR5/GPR49 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 697~726 amino acids from the loop2 region of human LGR5/GPR49.

Molecular Weight: 99867 DA

Comments:

Background: LGR5/GPR49 is an orphan receptor. It may be an important receptor for signals controlling growth and differentiation of specific embryonic tissues.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunofluorescence~~1:10~50 Immunohistochemistry ~~ 1:10~50 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Stem Cells, Cell Signaling, Cell Structure

Restrictions: For Research Use only

Images

LGR5/GPR49 Antibody (Loop2) (ABIN389218) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow)(Kindly offered by Dr. Li).

Western blot analysis of anti-LGR5/GPR49 Antibody (loop2) (ABIN389218) in mouse cerebellum tissue lysates (35ug/lane).LGR5/GPR49(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (loop2) (ABIN389218), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Barker, van Es, Kuipers et al.: "Identification of stem cells in small intestine and colon by marker gene Lgr5." in: Nature, Vol. 449, Issue 7165, pp. 1003-7, 2007 (PubMed).