Collagen, Type IX, alpha 1 (COL9A1) (Center) antibody

Antigen: Collagen, Type IX, alpha 1 (COL9A1)

Synonyms: MED, EDM6, FLJ40263, DJ149L1.1.2, Col9a-1, cb339, sb:cb339, zgc:85892, wu:fa12a12, wu:fa12d02, wu:fc22c08, wu:fc38d08, edm6, MGC82441, COL9A1

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389244

Additional Information

Alternative name: COL9A1

Gene ID: 1297

UniProt: P20849

Immunogen: This COL9A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 435~464 amino acids from the Center region of human COL9A1.

Isotype: Ig

Description: Other names: Collagen alpha-1(IX) chain [Precursor], COL9A1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This COL9A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 435~464 amino acids from the Center region of human COL9A1.

Molecular Weight: 91855 DA

Comments:

Background: COL9A1 is one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Studies in knockout mice have shown that synthesis of the alpha 1 chain is essential for assembly of type IX collagen molecules, a heterotrimeric molecule,and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in the COL9A1 gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-COL9A1 Antibody (Center) (ABIN389244) in 293 cell line lysates (35ug/lane). COL9A1(arrow) was detected using the purified Pab (1:60dilution).

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with COL9A1 antibody (Center) (ABIN389244), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Liu, Jin, Cao et al.: "[Analysis of association between COL9A1 gene and idiopathic congenital talipes equinovarus]." in: Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji, Vol. 29, Issue 4, pp. 427-32, 2007 (PubMed).

Fresquet, Jowitt, Ylöstalo et al.: "Structural and functional characterization of recombinant matrilin-3 A-domain and implications for human genetic bone diseases." in: The Journal of biological chemistry, Vol. 282, Issue 48, pp. 34634-43, 2007 (PubMed).