Heat Shock 70kDa Protein 14 (HSPA14) (Center) antibody

Antigen: Heat Shock 70kDa Protein 14 (HSPA14)

Synonyms: HSP70-4, HSP70L1, MGC131990

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389267

Additional Information

Alternative name: HSPA14

Gene ID: 51182

UniProt: Q0VDF9

Immunogen: This HSPA14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 234~264 amino acids from the Center region of human HSPA14.

Isotype: Ig

Description: Other names: Heat shock 70 kDa protein 14, Heat shock protein HSP60, HSP70-like protein 1, HSPA14, HSP60, HSP70L1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HSPA14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 234~264 amino acids from the Center region of human HSPA14.

Molecular Weight: 54794 DA

Comments:

Background: Hsp70-like protein 1 (Hsp70L1), a novel HSP derived from human dendritic cells (DCs), has potent adjuvant effects that polarize responses toward Th1.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cell Signaling

Restrictions: For Research Use only

Images

Western blot analysis of anti-HSPA14 Antibody (Center) (ABIN389267) in 293 cell line lysates (35ug/lane). HSPA14(arrow) was detected using the purified Pab.

Publications

Product: Scheufler, Brinker, Bourenkov et al.: "Structure of TPR domain-peptide complexes: critical elements in the assembly of the Hsp70-Hsp90 multichaperone machine." in: Cell, Vol. 101, Issue 2, pp. 199-210, 2000 (PubMed).

Wan, Zhou, Chen et al.: "Novel heat shock protein Hsp70L1 activates dendritic cells and acts as a Th1 polarizing adjuvant." in: Blood, Vol. 103, Issue 5, pp. 1747-54, 2004 (PubMed).