Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) (N-Term), (AA 1-30) antibody

Details for Product No. ABIN389281
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Antigen
Epitope
N-Term, AA 1-30
(7), (3), (3), (3), (2), (1)
Reactivity
Human
(39), (18), (17), (12), (12), (12)
Host
Rabbit
(34), (5)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Flow Cytometry (FACS)
(29), (10), (8), (4), (4), (4), (1), (1)
Pubmed 4 references available
Quantity 400 μL
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Catalog No. ABIN389281
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Immunogen This GADD45A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from the N-terminal region of human GADD45A.
Clone RB16520
Isotype Ig
Specificity This GADD45A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3~32 amino acids from the N-terminal region of human GADD45A.
Predicted Reactivity Cow (Bovine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name GADD45A
Background GADD45A responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45A gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms.
Synonyms: Growth arrest and DNA-damage-inducible protein GADD45 alpha, DNA-damage-inducible transcript 1, DDIT-1, GADD45A, DDIT1, GADD45
Molecular Weight 18205 DA
Gene ID 1647
UniProt P24522
Research Area Stem Cells, Protein Modifications
Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) (N-Term), (AA 1-30) antibody Western blot analysis of anti-GADD45A Antibody (N-term) (ABIN389281) in Y79 cell line lysates (35 µg/lane). GADD45A (arrow) was detected using the purified polyclonal antibody.
anti-Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) (N-Term), (AA 1-30) antibody (2) Western blot analysis of GADD45A (arrow) using rabbit polyclonal GADD45A Antibody (N-term) (ABIN389281). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GADD45A gene.
Background publications Kearsey, Coates, Prescott et al.: "Gadd45 is a nuclear cell cycle regulated protein which interacts with p21Cip1." in: Oncogene, Vol. 11, Issue 9, pp. 1675-83, 1995 (PubMed).

Zhu, Ren, Korchin et al.: "Soft tissue sarcoma cells are highly sensitive to AKT blockade: a role for p53-independent up-regulation of GADD45 alpha." in: Cancer research, Vol. 68, Issue 8, pp. 2895-903, 2008 (PubMed).

Al-Romaih, Sadikovic, Yoshimoto et al.: "Decitabine-induced demethylation of 5' CpG island in GADD45A leads to apoptosis in osteosarcoma cells." in: Neoplasia (New York, N.Y.), Vol. 10, Issue 5, pp. 471-80, 2008 (PubMed).

Li, Morales, Hwang et al.: "DNA mismatch repair-dependent activation of c-Abl/p73alpha/GADD45alpha-mediated apoptosis." in: The Journal of biological chemistry, Vol. 283, Issue 31, pp. 21394-403, 2008 (PubMed).

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