Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1) (N-Term,His44) antibody

Antigen: Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1)

Binding Site: N-Term,His44

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389296

Additional Information

Alternative name: RCHY1

Gene ID: 25898

UniProt: Q96PM5

Immunogen: This RCHY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37~66 amino acids from the N-terminal region of human RCHY1.

Isotype: Ig

Description: Other names: RING finger and CHY zinc finger domain-containing protein 1, Zinc finger protein 363, CH-rich-interacting match with PLAG1, Androgen receptor N-terminal-interacting protein, p53-induced RING-H2 protein, RING finger protein 199

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RCHY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37~66 amino acids from the N-terminal region of human RCHY1.

Molecular Weight: 30110 DA

Comments:

Background: RCHY1 protein has ubiquitin-protein ligase activity. This protein binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. Gene of RCHY1 is oncogenic because loss of p53 function contributes directly to malignant tumor development.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Stem Cells, Protein Modifications, Cancer, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-RCHY1 Antibody (N-term H44) (ABIN389296) in HL60 cell line lysates (35ug/lane). RCHY1(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with STK32C Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

RCHY1 Antibody (N-term H44) (ABIN389296) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Duan, Gao, Druhan et al.: "Expression of Pirh2, a newly identified ubiquitin protein ligase, in lung cancer." in: Journal of the National Cancer Institute, Vol. 96, Issue 22, pp. 1718-21, 2004 (PubMed).

Duan, Yao, Hou et al.: "Phosphorylation of Pirh2 by calmodulin-dependent kinase II impairs its ability to ubiquitinate p53." in: The EMBO journal, Vol. 26, Issue 13, pp. 3062-74, 2007 (PubMed).