NOP56 Ribonucleoprotein Homolog (Yeast) (NOP56) (N-Term) antibody

Antigen: NOP56 Ribonucleoprotein Homolog (Yeast) (NOP56)

Synonyms: NOL5A

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389361

Additional Information

Alternative name: NOL5A

Gene ID: 10528

UniProt: O00567

Immunogen: This NOL5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144~173 amino acids from the N-terminal region of human NOL5A.

Isotype: Ig

Description: Other names: Nucleolar protein Nop56, Nucleolar protein 5A, NOL5A, NOP56

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This NOL5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144~173 amino acids from the N-terminal region of human NOL5A.

Molecular Weight: 66050 DA

Comments:

Background: NOL5A is a yeast nucleolar protein that is part of a complex with the nucleolar proteins Nop58p and fibrillarin. This protein is required for assembly of the 60S ribosomal subunit and is involved in pre-rRNA processing. The protein is similar in sequence to Nop56p and is also found in the nucleolus.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells

Restrictions: For Research Use only

Images

Western blot analysis of anti-NOL5A Antibody (N-term) (ABIN389361) in NCI-H460 cell line lysates (35ug/lane). NOL5A(arrow) was detected using the purified Pab.

Publications

Product: Gautier, Bergès, Tollervey et al.: "Nucleolar KKE/D repeat proteins Nop56p and Nop58p interact with Nop1p and are required for ribosome biogenesis." in: Molecular and cellular biology, Vol. 17, Issue 12, pp. 7088-98, 1997 (PubMed).