NOP56 Ribonucleoprotein Homolog (Yeast) (NOP56) (Center) antibody

Antigen: NOP56 Ribonucleoprotein Homolog (Yeast) (NOP56)

Synonyms: NOL5A

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389362

Additional Information

Alternative name: NOL5A

Gene ID: 10528

UniProt: O00567

Immunogen: This NOL5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401~430 amino acids from the Center region of human NOL5A.

Isotype: Ig

Description: Other names: Nucleolar protein 5A, Nucleolar protein Nop56, NOL5A, NOP56

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This NOL5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401~430 amino acids from the Center region of human NOL5A.

Molecular Weight: 66050 DA

Comments:

Background: Nop56p is a yeast nucleolar protein that is part of a complex with the nucleolar proteins Nop58p and fibrillarin. Nop56p is required for assembly of the 60S ribosomal subunit and is involved in pre-rRNA processing. NOL5A is similar in sequence to Nop56p and is also found in the nucleolus.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells

Restrictions: For Research Use only

Images

Western blot analysis of anti-NOL5A Antibody (Center) (ABIN389362) in 293 cell line lysates (35ug/lane). NOL5A (arrow) was detected using the purified Pab.

Publications

Product: Hayano, Yanagida, Yamauchi et al.: "Proteomic analysis of human Nop56p-associated pre-ribosomal ribonucleoprotein complexes. Possible link between Nop56p and the nucleolar protein treacle responsible for Treacher Collins syndrome." in: The Journal of biological chemistry, Vol. 278, Issue 36, pp. 34309-19, 2003 (PubMed).

Nousiainen, Silljé, Sauer et al.: "Phosphoproteome analysis of the human mitotic spindle." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 14, pp. 5391-6, 2006 (PubMed).