Exosome Component 7 (EXOSC7) (C-Term) antibody

Antigen: Exosome Component 7 (EXOSC7)

Synonyms: p8, EAP1, RRP42, Rrp42p, hRrp42p, FLJ26543, KIAA0116, AV212732, mKIAA0116, 2610002K22Rik, exosc7, MGC81766, EXOSC7

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389375

Additional Information

Alternative name: EXOSC7

Gene ID: 23016

UniProt: Q15024

Immunogen: This EXOSC7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232~262 amino acids from the C-terminal region of human EXOSC7.

Isotype: Ig

Description: Other names: Exosome complex exonuclease RRP42, Ribosomal RNA-processing protein 42, Exosome component 7, p8, KIAA0116, RRP42

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This EXOSC7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232~262 amino acids from the C-terminal region of human EXOSC7.

Molecular Weight: 31835 DA

Comments:

Background: EXOSC7 is a component of the exosome 3'->5' exoribonuclease complex, a complex that degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3'-untranslated regions. The protein is required for the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA and has a 3'-5' exonuclease activity.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Translation Factors, Stem Cells, Signaling

Restrictions: For Research Use only

Images

Western blot analysis of EXOSC7 Antibody (C-term) (ABIN389375) in K562, NCI-H460 cell line lysates (35ug/lane). EXOSC7 (arrow) was detected using the purified Pab.

Western blot analysis of EXOSC7 (arrow) using rabbit polyclonal EXOSC7 Antibody (C-term) (ABIN389375). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EXOSC7 gene.

Publications

Product: Chen, Gherzi, Ong et al.: "AU binding proteins recruit the exosome to degrade ARE-containing mRNAs." in: Cell, Vol. 107, Issue 4, pp. 451-64, 2001 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).