Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK) (N-Term) antibody

Antigen: Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK)

Synonyms: CSBP, TUNP, HNRPK, FLJ41122, Csbp, Hnrpk, hnrpk, MGC53459, MGC75642, zgc:66162, wu:fb37h02, wu:fi34c04, HNRNPK, MGC127613, DKFZp469J0720, KBBP, NOVA, MGC102032

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389377

Additional Information

Alternative name: HNRPK

Gene ID: 3190

UniProt: P61978

Immunogen: This HNRPK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human HNRPK.

Isotype: Ig

Description: Other names: Heterogeneous nuclear ribonucleoprotein K, Transformation up-regulated nuclear protein, HNRPK

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HNRPK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human HNRPK.

Molecular Weight: 42593 DA

Comments:

Background: HNRPK belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. HNRPK is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. The protein is distinct among other hnRNP proteins in its binding preference, it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Stem Cells, Signaling

Restrictions: For Research Use only

Images

Western blot analysis of anti-HNRPK Antibody (N-term) (ABIN389377) in 293 cell line lysates (35ug/lane). HNRPK(arrow) was detected using the purified Pab.

Western blot analysis of anti-HNRPK Antibody (N-term) (ABIN389377) in mouse brain tissue lysates (35ug/lane). HNRPK(arrow) was detected using the purified Pab.

Publications

Product: Hsieh, Matsumoto, Chou et al.: "Hepatitis C virus core protein interacts with heterogeneous nuclear ribonucleoprotein K." in: The Journal of biological chemistry, Vol. 273, Issue 28, pp. 17651-9, 1998 (PubMed).