Heat Shock 70kDa Protein 2 (HSPA2) (C-Term,Cys577) antibody

Antigen: Heat Shock 70kDa Protein 2 (HSPA2)

Synonyms: HSP70-2, HSP70-3, HSPA2, Hspa2, DKFZp468B217

Binding Site: C-Term,Cys577

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389379

Additional Information

Alternative name: HSPA2

Gene ID: 3306

UniProt: P54652

Immunogen: This HSPA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 572~601 amino acids from the C-terminal region of human HSPA2.

Isotype: Ig

Description: Other names: Heat shock-related 70 kDa protein 2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HSPA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 572~601 amino acids from the C-terminal region of human HSPA2.

Molecular Weight: 70021 DA

Comments:

Background: In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Stem Cells, Cell Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of HSPA2 Antibody (C-term C577) (ABIN389379) in Hela, A2058 and A375 cell line lysates (35ug/lane). HSPA2 (arrow) was detected using the purified Pab.

Western blot analysis of HSPA2 (arrow) using rabbit polyclonal HSPA2 Antibody (C-term C577) (ABIN389379). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSPA2 gene.

Publications

Product: Roux, Nguyen, Squire et al.: "A heat shock gene at 14q22: mapping and expression." in: Human molecular genetics, Vol. 3, Issue 10, pp. 1819-22, 1995 (PubMed).

Rikova, Guo, Zeng et al.: "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." in: Cell, Vol. 131, Issue 6, pp. 1190-203, 2007 (PubMed).