COP9 Constitutive Photomorphogenic Homolog Subunit 6 (Arabidopsis) (COPS6) (Center) antibody

Antigen: COP9 Constitutive Photomorphogenic Homolog Subunit 6 (Arabidopsis) (COPS6)

Synonyms: CSN6, MOV34-34KD, MGC159502, zgc:112075, DKFZp468M0916

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389395

Additional Information

Alternative name: COPS6

Gene ID: 10980

UniProt: Q7L5N1

Immunogen: This COPS6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122~152 amino acids from the Center region of human COPS6.

Isotype: Ig

Description: Other names: COP9 signalosome complex subunit 6, JAB1-containing signalosome subunit 6, Vpr-interacting protein, MOV34 homolog, CSN6, HVIP

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This COPS6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122~152 amino acids from the Center region of human COPS6.

Molecular Weight: 36163 DA

Comments:

Background: COPS6 is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. This protein belongs to translation initiation factor 3 (eIF3) superfamily. It is involved in the regulation of cell cycle and likely to be a cellular cofactor for HIV-1 accessory gene product Vpr.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-COPS6 Antibody (Center) (ABIN389395) in NCI-H460 cell line lysates (35ug/lane). COPS6(arrow) was detected using the purified Pab.

Publications

Product: Zhao, Mukherjee, Narayan: "Biochemical mechanism of HIV-I Vpr function. Specific interaction with a cellular protein." in: The Journal of biological chemistry, Vol. 269, Issue 22, pp. 15577-82, 1994 (PubMed).

Mahalingam, Ayyavoo, Patel et al.: "HIV-1 Vpr interacts with a human 34-kDa mov34 homologue, a cellular factor linked to the G2/M phase transition of the mammalian cell cycle." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3419-24, 1998 (PubMed).

Hoareau Alves, Bochard, Réty et al.: "Association of the mammalian proto-oncoprotein Int-6 with the three protein complexes eIF3, COP9 signalosome and 26S proteasome." in: FEBS letters, Vol. 527, Issue 1-3, pp. 15-21, 2002 (PubMed).

Groisman, Polanowska, Kuraoka et al.: "The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage." in: Cell, Vol. 113, Issue 3, pp. 357-67, 2003 (PubMed).