Heat Shock 10kDa Protein 1 (Chaperonin 10) (HSPE1) (N-Term) antibody

Antigen: Heat Shock 10kDa Protein 1 (Chaperonin 10) (HSPE1)

Synonyms: CPN10, GROES, HSP10, groS, Cpn10, cpn10, zgc:86747, HSPE1, MGC79030, hspe1, MGC89647, CHLOROPLAST CHAPERONIN 10, F16B22.14, ATCPN21, CHAPERONIN 10, CHAPERONIN 20, CHCPN10, CPN20, CPN21, T1M15.120, T1M15_120, MITOCHONDRIAL CHAPERONIN 10, T15D22.2, T15D22_2, anon-WO0118547.175, DmelCG11267, Hsp10, hsp10, crpB, chpS

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389396

Additional Information

Alternative name: HSPE1

Gene ID: 3336

UniProt: P61604

Immunogen: This HSPE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-term region of human HSPE1.

Isotype: Ig

Description: Other names: 10 kDa heat shock protein, mitochondrial, 10 kDa chaperonin, CPN10, Early-pregnancy factor

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HSPE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-term region of human HSPE1.

Molecular Weight: 10932 DA

Comments:

Background: HSPE1 is a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner.

Synonyms: CPN10, GROES, HSP10, groS, Cpn10, cpn10, zgc:86747, HSPE1, MGC79030, hspe1, MGC89647, CHLOROPLAST CHAPERONIN 10, F16B22.14, ATCPN21, CHAPERONIN 10, CHAPERONIN 20, CHCPN10, CPN20, CPN21, T1M15.120, T1M15_120, MITOCHONDRIAL CHAPERONIN 10, T15D22.2, T15D22_2, anon-WO0118547.175, DmelCG11267, Hsp10, hsp10, crpB, chpS

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells

Restrictions: For Research Use only

Images

Western blot analysis of anti-HSPE1 Antibody (N-term) (ABIN389396) in HL60 cell line lysates (35ug/lane). HSPE1(arrow) was detected using the purified Pab.

Publications

Product: Cavanagh, Morton: "The purification of early-pregnancy factor to homogeneity from human platelets and identification as chaperonin 10." in: European journal of biochemistry / FEBS, Vol. 222, Issue 2, pp. 551-60, 1994 (PubMed).

Chen, McNealy, Dalal et al.: "Isolation, sequence analysis and characterization of a cDNA encoding human chaperonin 10." in: Biochimica et biophysica acta, Vol. 1219, Issue 1, pp. 189-90, 1994 (PubMed).

Hansen, Bross, Westergaard et al.: "Genomic structure of the human mitochondrial chaperonin genes: HSP60 and HSP10 are localised head to head on chromosome 2 separated by a bidirectional promoter." in: Human genetics, Vol. 112, Issue 1, pp. 71-7, 2002 (PubMed).