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Heat Shock 70kDa Protein 8 (HSPA8) (C-Term) antibody

Antigen

Heat Shock 70kDa Protein 8 (HSPA8)

Synonyms LAP1, HSC54, HSC70, HSC71, HSP71, HSP73, NIP71, HSPA10, MGC29929, MGC131511
Binding Site
Alternatives

C-Term

Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human

Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), ELISA
1 reference available
Certificates ISO 9001:2008
Catalog no. ABIN389398
Quantity 400 µL  (0.25 mg/ml)
Price 280.50 $   Plus shipping costs $45.00
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Additional Information

Alternative name HSPA8
Gene ID 3312
UniProt P11142
Immunogen This HSPA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 547~576 amino acids from the C-terminal region of human HSPA8.
Isotype Ig
Description Other names: Heat shock cognate 71 kDa protein, Heat shock 70 kDa protein 8, synonyms: HSPA10, synonyms: HSP73, synonyms: HSC70, name: HSPA8,
Characteristics Purified Rabbit Polyclonal Antibody (Pab)
Specificity This HSPA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 547~576 amino acids from the C-terminal region of human HSPA8.
Molecular Weight 70898 DA
Comments

Background: HSPA8 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 is a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. The protein also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell.

Application Details

Application Notes The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100
Concentration 0.25 mg/ml
Purification Purified
Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles
Research Area Stem Cells, Cell Signaling, Cell Structure
Restrictions For Research Use only

Publications

Product Egerton, Moritz, Druker et al.: "Identification of the 70kD heat shock cognate protein (Hsc70) and alpha-actinin-1 as novel phosphotyrosine-containing proteins in T lymphocytes." in: Biochemical and biophysical research communications, Vol. 224, Issue 3, pp. 666-74, 1996 (PubMed).

Alternatives

Alternatives for antigen "Heat Shock 70kDa Protein 8 (HSPA8)", type "Antibodies"
Hosts Rabbit (73), Mouse (24), Goat (9), Rat (9)
Reactivities Human (80), Rat (Rattus) (55), Mouse (Murine) (50), Cow (Bovine) (29), Chicken (28), Pig (Porcine) (23), Hamster (16), Dog (Canine) (9), Rabbit (5), Sheep (Ovine) (5), Fruit Fly (Drosophila melanogaster) (4), Monkey (4), Fish (3), Guinea Pig (3), Spinach (Spinacia oleracea) (3), Xenopus laevis (3), Arabidopsis (2), Caenorhabditis elegans (C. elegans) (2), Pea (Pisum sativum) (2), Wheat (Triticum) (2), Yeast (2), Cat (Feline) (1), Mycobacteria (1), Plant (1), Squash (Cucurbita) (1), Wheat germ (1), Yeast (Pichia pastoris) (1)
Applications Western Blotting (WB) (95), ELISA (51), Immunohistochemistry (IHC) (43), Immunoprecipitation (IP) (35), Immunofluorescence (IF) (29), Immunocytochemistry (ICC) (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Flow Cytometry (FACS) (7), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Functional Studies (Func) (2), Blocking Antibody (Inhibition) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1)
Conjugates Biotin (8), FITC (5), PE (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes Internal Region (10), Isoform 1 (3), N-Term (3), AA 650-670 (2), AA 534-615 (1), AA 556-568 (1), AA 618-637 (1), C-Term (1)