Actin, Alpha, Cardiac Muscle 1 (ACTC1) (C-Term) antibody

Antigen: Actin, Alpha, Cardiac Muscle 1 (ACTC1)

Synonyms: ACTC, ASD5, CMD1R, CMH11, MGC156490, actcl, actc1l, ACTC1, actc, cmd1r, MGC75679, ACTBL2, MGC128362, ACTA, ASMA, CFTD, MPFD, NEM1, NEM2, NEM3, CFTD1, CFTDM, Acts, Acta-2, Actsk-1, AA959943, fa27h01, fb83f06, wu:fa27h01, wu:fb83f06, ACTA1, acta1, MGC52636, MGC79039, ACTB, acta, asma, cftd, mpfd, nem1, nem2, nem3, acta2, cftd1, cftdm, MGC89192, ACTG1, LOC100154254, DKFZp468G072, Actc-1

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389401

Additional Information

Alternative name: ACTC

Gene ID: 59

UniProt: P62736

Immunogen: This ACTC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 348~377 amino acids from the C-terminal region of human ACTC.

Isotype: Ig

Description: Other names: Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, GIG46, ACTVS, ACTSA, ACTA2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ACTC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 348~377 amino acids from the C-terminal region of human ACTC.

Molecular Weight: 42009 DA

Comments:

Background: ACTC belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this protein cause aortic aneurysm familial thoracic type 6.

Synonyms: ACTC, ASD5, CMD1R, CMH11, MGC156490, actcl, actc1l, ACTC1, actc, cmd1r, MGC75679, ACTBL2, MGC128362, ACTA, ASMA, CFTD, MPFD, NEM1, NEM2, NEM3, CFTD1, CFTDM, Acts, Acta-2, Actsk-1, AA959943, fa27h01, fb83f06, wu:fa27h01, wu:fb83f06, ACTA1, acta1, MGC52636, MGC79039, ACTB, acta, asma, cftd, mpfd, nem1, nem2, nem3, acta2, cftd1, cftdm, MGC89192, ACTG1, LOC100154254, DKFZp468G072, Actc-1

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ACTC antibody (C-term) (ABIN389401) in mouse heart tissue lysates (35ug/lane). ACTC (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human skeletal muscle with ACTC Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Flow cytometric analysis of NCI-H292 cells using ACTC Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Guo, Pannu, Tran-Fadulu et al.: "Mutations in smooth muscle alpha-actin (ACTA2) lead to thoracic aortic aneurysms and dissections." in: Nature genetics, Vol. 39, Issue 12, pp. 1488-93, 2007 (PubMed).

Guo, Papke, Tran-Fadulu et al.: "Mutations in smooth muscle alpha-actin (ACTA2) cause coronary artery disease, stroke, and Moyamoya disease, along with thoracic aortic disease." in: American journal of human genetics, Vol. 84, Issue 5, pp. 617-27, 2009 (PubMed).