ACTC (Center) antibody

Antigen: ACTC

Epitope: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389402

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: ACTC (Center)

Gene ID: 59

Swiss-Prot: P62736

Immunogen: This ACTC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 197~227 amino acids from the Center region of human ACTC.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, GIG46, ACTVS, ACTSA, ACTA2

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human ACTC.

Molecular Weight: 42009 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ACTC antibody (Center)(ABIN389402) in K562 cell line lysates (35ug/lane). ACTC (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human skeletal muscle with ACTC Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Flow cytometric analysis of MDA-231 cells using ACTC Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.