Chaperonin Containing TCP1, Subunit 2 (Beta) (CCT2) (N-Term) antibody

Antigen: Chaperonin Containing TCP1, Subunit 2 (Beta) (CCT2)

Synonyms: CCTB, 99D8.1, PRO1633, CCT-beta, MGC142074, MGC142076, TCP-1-beta, fa26a01, fc58a11, CHUNP6883, wu:fa26a01, wu:fc58a11, cct2, MGC89448, CCT2, MGC128852, Cctb, MGC94480

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389419

Additional Information

Alternative name: CCT2

Gene ID: 10576

UniProt: P78371

Immunogen: This CCT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5~35 amino acids from the N-terminal region of human CCT2.

Isotype: Ig

Description: Other names: T-complex protein 1 subunit beta, TCP-1-beta, CCT-beta, CCTB, 99D8.1, CCT2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CCT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5~35 amino acids from the N-terminal region of human CCT2.

Molecular Weight: 57488 DA

Comments:

Background: CCT2 is a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Protein Modifications

Restrictions: For Research Use only

Images

Western blot analysis of CCT2 Antibody (N-term) (ABIN389419) in A2058 cell line lysates (35ug/lane). CCT2 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with CCT2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

CCT2 Antibody (N-term) (ABIN389419) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Zebol, Hewitt, Moretti et al.: "The CCT/TRiC chaperonin is required for maturation of sphingosine kinase 1." in: The international journal of biochemistry & cell biology, Vol. 41, Issue 4, pp. 822-7, 2009 (PubMed).

Abe, Yoon, Kubota et al.: "p90 ribosomal S6 kinase and p70 ribosomal S6 kinase link phosphorylation of the eukaryotic chaperonin containing TCP-1 to growth factor, insulin, and nutrient signaling." in: The Journal of biological chemistry, Vol. 284, Issue 22, pp. 14939-48, 2009 (PubMed).