AHA1, Activator of Heat Shock 90kDa Protein ATPase Homolog 1 (Yeast) (AHSA1) (N-Term) antibody

Antigen: AHA1, Activator of Heat Shock 90kDa Protein ATPase Homolog 1 (Yeast) (AHSA1)

Synonyms: ahsa1, MGC52884, MGC132147, MGC80312, AHSA1, MGC89589, MGC136821, wu:fb83b05, zgc:136821

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389425

Additional Information

Alternative name: AHSA1

Gene ID: 10598

UniProt: O95433

Immunogen: This AHSA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human AHSA1.

Isotype: Ig

Description: Other names: Activator of 90 kDa heat shock protein ATPase homolog 1, AHA1, p38 ,HSPC322, C14orf3, AHSA1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This AHSA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human AHSA1.

Molecular Weight: 38274 DA

Comments:

Background: AHSA1 is a cochaperone that stimulates HSP90 ATPase activity. This protein may affect a step in the endoplasmic reticulum to Golgi trafficking.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells

Restrictions: For Research Use only

Images

Western blot analysis of AHSA1 Antibody (N-term) (ABIN389425) in HL-60, T47D cell line lysates (35ug/lane). AHSA1 (arrow) was detected using the purified Pab.

AHSA1 Antibody (N-term) (ABIN389425) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Panaretou, Siligardi, Meyer et al.: "Activation of the ATPase activity of hsp90 by the stress-regulated cochaperone aha1." in: Molecular cell, Vol. 10, Issue 6, pp. 1307-18, 2002 (PubMed).

Wang, Venable, LaPointe et al.: "Hsp90 cochaperone Aha1 downregulation rescues misfolding of CFTR in cystic fibrosis." in: Cell, Vol. 127, Issue 4, pp. 803-15, 2006 (PubMed).