Capping Protein (Actin Filament) Muscle Z-Line, alpha 2 (CAPZA2) (Center) antibody

Antigen: Capping Protein (Actin Filament) Muscle Z-Line, alpha 2 (CAPZA2)

Synonyms: CAPZ, CAPPA2, CAPZA2, DKFZp459K053

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389430

Additional Information

Alternative name: CAPZA2

Gene ID: 830

UniProt: P47755

Immunogen: This CAPZA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89~119 amino acids from the Center region of human CAPZA2.

Isotype: Ig

Description: Other names: F-actin-capping protein subunit alpha-2, CapZ alpha-2, CAPZA2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CAPZA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89~119 amino acids from the Center region of human CAPZA2.

Molecular Weight: 32949 DA

Comments:

Background: CAPZA2 is a member of the F-actin capping protein alpha subunit family. It is the alpha subunit of the barbed-end actin binding protein Cap Z. By capping the barbed end of actin filaments, Cap Z regulates the growth of the actin filaments at the barbed end.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells

Restrictions: For Research Use only

Images

Western blot analysis of CAPZA2 Antibody (Center) (ABIN389430) in T47D cell line lysates (35ug/lane). CAPZA2 (arrow) was detected using the purified Pab.

Western blot analysis of CAPZA2 Antibody (Center) (ABIN389430) in mouse spleen tissue lysates (35ug/lane). CAPZA2 (arrow) was detected using the purified Pab.

Western blot analysis of CAPZA2 (arrow) using rabbit polyclonal CAPZA2 Antibody (Center) (ABIN389430). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAPZA2 gene (Lane 2) .

Publications

Product: Miyagawa, Tanaka, Iguchi et al.: "Molecular cloning and characterization of the human orthologue of male germ cell-specific actin capping protein alpha3 (cpalpha3)." in: Molecular human reproduction, Vol. 8, Issue 6, pp. 531-9, 2002 (PubMed).

Denoeud, Kapranov, Ucla et al.: "Prominent use of distal 5' transcription start sites and discovery of a large number of additional exons in ENCODE regions." in: Genome research, Vol. 17, Issue 6, pp. 746-59, 2007 (PubMed).