Eukaryotic Translation Initiation Factor 5A (EIF5A) (Center) antibody

Antigen: Eukaryotic Translation Initiation Factor 5A (EIF5A)

Synonyms: EIF-5A, EIF5A1, eIF5AI, MGC99547, MGC104255, Eif4d, Eif5a1, MGC6725, AA410058, D19Wsu54e, MGC124873, zgc:77099, wu:fb17e01, wu:fb63h04, wu:fk81e04, EIF5A, zgc:55504, zgc:77429, wu:fb05b06, wu:fc23f04, wu:fc61c10, MGC128860

Epitope: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389438

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: EIF5A (Center)

Gene ID: 1984

Swiss-Prot: P63241

Immunogen: This EIF5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52~81 amino acids from the Center region of human EIF5A.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, eIF-5A, eIF-4D, Rev-binding factor , EIF5A

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human EIF5A.

Molecular Weight: 16832 DA

Synonyms: EIF-5A, EIF5A1, eIF5AI, MGC99547, MGC104255, Eif4d, Eif5a1, MGC6725, AA410058, D19Wsu54e, MGC124873, zgc:77099, wu:fb17e01, wu:fb63h04, wu:fk81e04, EIF5A, zgc:55504, zgc:77429, wu:fb05b06, wu:fc23f04, wu:fc61c10, MGC128860

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells

Restrictions: For Research Use only

Images

Western blot analysis of EIF5A Antibody (Center) (ABIN389438) in MDA-MB231 and MCF-7 cell line lysates (35ug/lane). EIF5A (arrow) was detected using the purified Pab.

Western blot analysis of EIF5A (arrow) using rabbit polyclonal EIF5A Antibody (Center) (ABIN389438). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF5A gene (Lane 2).

Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with EIF5A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.