Eukaryotic Translation Elongation Factor 2 (EEF2) (N-Term) antibody

Antigen: Eukaryotic Translation Elongation Factor 2 (EEF2)

Synonyms: eef2, MGC76191, MGC79628, EEF2, DKFZp468K065, DKFZp468M076

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN389444

Additional Information

Alternative name: EEF2

Gene ID: 1938

UniProt: P13639

Immunogen: This EEF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45~75 amino acids from the N-terminal region of human EEF2.

Isotype: Ig

Description: Other names: Elongation factor 2, EF-2, EF2, EEF2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This EEF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45~75 amino acids from the N-terminal region of human EEF2.

Molecular Weight: 95338 DA

Comments:

Background: EEF2 is a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells

Restrictions: For Research Use only

Images

Western blot analysis of EEF2 Antibody (N-term) (ABIN389444) in mouse NIH-3T3 tissue lysates (35ug/lane). EEF2 (arrow) was detected using the purified Pab.

Western blot analysis of EEF2 Antibody (N-term) (ABIN389444) in Y79 cell line lysates (35ug/lane). EEF2 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with EEF2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Redpath, Price, Severinov et al.: "Regulation of elongation factor-2 by multisite phosphorylation." in: European journal of biochemistry / FEBS, Vol. 213, Issue 2, pp. 689-99, 1993 (PubMed).

Nakamura, Aoyagi, Nanchi et al.: "Overexpression of eukaryotic elongation factor eEF2 in gastrointestinal cancers and its involvement in G2/M progression in the cell cycle." in: International journal of oncology, Vol. 34, Issue 5, pp. 1181-9, 2009 (PubMed).